FastQCFastQC Report
Sat 15 Jun 2019
HK7YLAFXY_n01_CRISPRi_tubeA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7YLAFXY_n01_CRISPRi_tubeA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18139739
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG278590.15357993849856386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGA2010750.0144.80492
TAACAAT1440750.0144.798741
ATACACG2011300.0144.750381
ATAGGTC1457000.0144.69092
ACACGAT2013300.0144.634743
ATCGTTA2588200.0144.595874
ACTGTAT1251550.0144.558013
TAAGTAG1893500.0144.554891
TCGTTAC2590000.0144.470175
TGTATCT1253350.0144.43725
TACTGTA1253050.0144.420062
CACGATC2014950.0144.415544
TAGAGTC1897450.0144.377665
TCGATAA1120700.0144.373813
ATTCCTT1345050.0144.353445
TCGATTC1345000.0144.32762
ATCTCTT3278600.0144.313668
ACGATCT2018850.0144.305365
TCGATAC1147000.0144.30034
TAGGTCG1456600.0144.220843
Produced by FastQC (version 0.11.5)