FastQCFastQC Report
Sat 15 Jun 2019
HK7YLAFXY_n01_CRISPRa_tubeB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7YLAFXY_n01_CRISPRa_tubeB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744917
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATAC151.2296991E-4145.013674
ATCGTTC253.7105565E-8145.013664
GTCTTTT100.0070911353145.00129
ATAGGTC149250.0144.924822
ATCGATT125750.0144.861451
TCGATCG243550.0144.826021
TAGGTCG149100.0144.721883
TAAGTAG190500.0144.691851
ATCGTTA243800.0144.686524
TACTGTA121550.0144.60442
TCATGAT120200.0144.591424
ATGATCG119900.0144.590356
TCGTTAC243800.0144.567575
ATAACGC110650.0144.489446
TAACAAT144550.0144.482621
ATACACG195300.0144.440351
ACGATCG118100.0144.420431
ACGATCT195200.0144.419345
TAGAGTC191250.0144.369175
TAACGCA110700.0144.342157
Produced by FastQC (version 0.11.5)