FastQCFastQC Report
Sat 15 Jun 2019
HK7YLAFXY_n01_CRISPRa_tubeA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7YLAFXY_n01_CRISPRa_tubeA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3323244
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTAG355850.0144.761291
TAGGTCG271600.0144.736223
TCGATCG466950.0144.67871
ATAGGTC272400.0144.639312
TCGATTC248900.0144.630742
TAACAAT275100.0144.618821
ACGATCG224600.0144.582631
ATGATCG234300.0144.565676
AAGTAGA356600.0144.550582
TCGCATC271750.0144.541477
ATCGTTA468300.0144.534414
ATCGATT248800.0144.51311
ATACACG372950.0144.444671
TCGTTAC467950.0144.441075
CGATCGT468400.0144.419372
ACGATCT373450.0144.369725
GTCGCAT272150.0144.36386
TAGGTAA224150.0144.31589
CACGATC373100.0144.310794
ACTGTAT233200.0144.283623
Produced by FastQC (version 0.11.5)