FastQCFastQC Report
Fri 4 Aug 2023
HK7N5DMXY_n02_CIVR_Y4_D7_P2_G10_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7N5DMXY_n02_CIVR_Y4_D7_P2_G10_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171574
Sequences flagged as poor quality0
Sequence length151
%GC77

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCACCGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG2050111.948780118199727Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60143.5051930945248118No Hit
CTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACAATAG20451.1919055334724376Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACAATAGTGTAGATC20031.1674263000221479Illumina Single End PCR Primer 1 (96% over 33bp)
CCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACAATAGTGT14060.8194714816930303Illumina Single End PCR Primer 1 (96% over 33bp)
ACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACAATA8780.5117325468893887Illumina Single End PCR Primer 1 (96% over 33bp)
ATCACCGACTGCCCATAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTG7260.4231410353550072Illumina Single End PCR Primer 1 (96% over 33bp)
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATAGATCGGAA6210.361942951729283ABI Solid3 Adapter B (100% over 41bp)
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGAGATCGGAA6200.36136011283760944ABI Solid3 Adapter B (100% over 41bp)
CGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACAA5820.339212234954014Illumina Single End PCR Primer 1 (96% over 33bp)
GCCTTAATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCC4720.275099956869922No Hit
GCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACAATAGTG4570.26635737349481853Illumina Single End PCR Primer 1 (96% over 33bp)
ATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCCTCGATG4560.265774534603145No Hit
ATCACCGAACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT4300.2506207234196323Illumina Single End PCR Primer 1 (100% over 33bp)
ATCACCGGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT3400.19816522316901164Illumina Single End PCR Primer 1 (100% over 33bp)
GACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACAAT3350.1952510287106438Illumina Single End PCR Primer 1 (96% over 33bp)
CTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACAATA3300.192336834252276Illumina Single End PCR Primer 1 (96% over 33bp)
CCGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACA3130.1824285730938254Illumina Single End PCR Primer 1 (96% over 33bp)
TGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACAATAGT2780.16202921188525068Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACAATA2750.16028069521022997Illumina Single End PCR Primer 1 (96% over 33bp)
CCTTAATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCCT2660.1550351451851679No Hit
CCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACAATAGTGTA2570.14978959516010584Illumina Single End PCR Primer 1 (96% over 33bp)
ATCACGGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG2370.13813281732663457Illumina Single End PCR Primer 1 (96% over 33bp)
ATCACCGACTGCCCATAGAGAGGAGATCGGAAGAGCGTCGTGTAGGGAAA2140.12472752281814262Illumina Single End PCR Primer 1 (100% over 27bp)
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACAATAGTGTAGA1760.10257964493454719Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)