FastQCFastQC Report
Mon 1 May 2023
HK7N5DMXY_n02_CIVR_Y4_D7_P2_F3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7N5DMXY_n02_CIVR_Y4_D7_P2_F3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42453839
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC2641240.6221439714792342No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA2476190.5832664508856313No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC2207020.5198634686488541No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT1962710.4623162583718283No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC1570430.3699147207864994No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC1317670.31037711336305773No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA1201040.2829049217433552No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT1188560.27996525826557167No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA1179550.27784295314258856No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG1175260.2768324438221005No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG1043070.2456950948534949No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA1021100.24052006227281353No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC1012020.23838126865275952No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGAC972690.22911708879849477No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG936450.2205807583149312No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC893100.2103696676288804No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC888990.20940155730086035No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA804740.18955647332624032No Hit
CTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGAT799700.18836930153713544No Hit
GTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAA765940.18041713494979808No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC763900.17993661303516037No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC759020.17878712923936044No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT722600.17020839976332883No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT712870.1679164986704736No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC709430.1671062068144179No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT701070.16513700916423601No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG679450.16004441906890918No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC650360.15319227078615905No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC649810.15306271830917342No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC647330.1524785544129472No Hit
GTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCA639940.15073784022217637No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT635430.14967550991089404No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC633060.14911725651006497No Hit
TGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACC616590.14523774869924014No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA603380.14212613375200298No Hit
GGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCA571100.13452258110273607No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA568930.13401143769353815No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA557510.13132145717139973No Hit
GTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCT554080.1305135208149256No Hit
GGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGG554040.13050409881659936No Hit
CGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGA547150.1288811596049064No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC545300.1284453921823183No Hit
CGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCG540800.12738541737061754No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA534910.1259980281170803No Hit
GGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGC524310.12350119856062959No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT517510.12189945884517063No Hit
GAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCA493220.11617795036156801No Hit
CGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACC489260.11524517252727133No Hit
CAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATG488650.11510148705279634No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG488600.11508970955488855No Hit
CCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTAT487640.11486358159505906No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG483190.11381538428126606No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG474940.1118720971264813No Hit
CTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGC474540.111777877143219No Hit
CAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGT473760.11159414817585754No Hit
CTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTT471930.11116309175243255No Hit
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACA470560.11084038830975922No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA468760.11041639838507891No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT466910.10998063096249082No Hit
GGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCT460060.10836711374912408No Hit
GCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTC459770.10829880426125893No Hit
CAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCG450500.10611525614915532No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC450390.10608934565375819No Hit
GCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTA447410.10538740677845412No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGA446490.10517070081695085No Hit
CGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTG441650.10403063901947714No Hit
CTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGA438840.10336874363705954No Hit
GCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCG429960.10127706000863668No Hit
GTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGC429090.10107213154504119No Hit
GCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGG428650.10096848956345267No Hit
CAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCC428390.10090724657433219No Hit
GCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCA425210.10015819770739698No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)