FastQCFastQC Report
Fri 4 Aug 2023
HK7N5DMXY_n02_CIVR_Y4_D7_P2_D6_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7N5DMXY_n02_CIVR_Y4_D7_P2_D6_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126784
Sequences flagged as poor quality0
Sequence length151
%GC83

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70345.548018677435638No Hit
ATCACCGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC56434.450877082281676Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT17431.374779151943463No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGTGCAAGTGTAGATC16381.2919611307420495Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT15131.1933682483594146No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG14271.1255363452801614No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG8780.6925164058556285No Hit
CTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGTGCAAG7050.5560638566380616Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG6040.47640080767289245No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG5350.42197753659767795No Hit
CCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGTGCAAGTGT5320.41961130742049474Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTG5300.4180338213023725No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGT5110.403047703180212No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTG4870.3841178697627461No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTT4770.3762304391721353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG4610.363610550227158No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTGT4540.35808934881373045No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTT4410.34783568904593637No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGT4340.3423144876325088No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTGG3900.3076097930338213No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGT3890.30682104997476023No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGG3600.28394750126198887No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG3050.2405666330136295No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTG2880.2271580010095911No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTG2800.2208480565371025No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTT2750.21690434124179708No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGG2730.21532685512367492No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGT2730.21532685512367492No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTT2700.21296062594649165No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGT2680.2113831398283695No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGTG2640.20822816759212517No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGTGT2490.19639702170620899No Hit
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGAGATCGGAA2280.1798334174659263ABI Solid3 Adapter B (100% over 41bp)
ACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGTGCAA2240.17667844522968199Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGT2220.17510095911155982No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTTG2080.1640585562847047No Hit
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATAGATCGGAA2050.16169232710752146ABI Solid3 Adapter B (100% over 41bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTTT1970.15538238263503282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGGG1920.1514386673397274No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGG1920.1514386673397274No Hit
ATCACCGACTGCCCATAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTC1840.14512872286723877Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTGT1830.14433997980817767No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGTGT1780.1403962645128723No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG1720.13566380615850582No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTG1700.13408632004038365No Hit
CGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGTGC1650.13014260474507824Illumina Single End PCR Primer 1 (97% over 35bp)
ATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCCTCGATG1620.127776375567895No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGTG1610.1269876325088339No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGTG1570.1238326602725896No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGTGT1540.12146643109540636No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGGT1520.11988894497728421No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTTT1520.11988894497728421No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTTG1500.11831145885916204No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGTGG1480.11673397274103987No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGTT1440.11357900050479555No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGG1420.11200151438667338No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGTGG1420.11200151438667338No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGT1400.11042402826855124No Hit
GCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGTGCAAGTG1340.10569156991418475Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTGG1300.10253659767794045No Hit
GCCTTAATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCC1290.10174785461887935No Hit
ATCACCGAACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT1280.10095911155981827Illumina Single End PCR Primer 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)