FastQCFastQC Report
Mon 1 May 2023
HK7N5DMXY_n02_CIVR_Y4_D7_P1_D6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7N5DMXY_n02_CIVR_Y4_D7_P1_D6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15659
Sequences flagged as poor quality0
Sequence length151
%GC71

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCACCAGTGTAGATC8225.24937735487579Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5833.7230985375822208No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2041.302765182961875Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC1651.0537071332779873Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1220.7791046682419056Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG1080.689699214509228Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA700.44702726866338843Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG610.3895523341209528Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG610.3895523341209528Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG520.3320773995785171Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA460.2937607765502267Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG450.2873746727121783Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG420.2682163611980331Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT420.2682163611980331No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT410.26183025735998466No Hit
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCG410.26183025735998466No Hit
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTC350.22351363433169422No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG350.22351363433169422No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT330.21074142665559742No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT310.1979692189795006No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTT310.1979692189795006Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATATATATATATATATATAAGATCG290.1851970113034038No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG280.17881090746535538Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATATATATATATATATAAGATCGGAAGAG250.15965259595121017No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT230.14688038827511335No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG220.14049428443706496No Hit
GATCGGAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCACCA220.14049428443706496Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATATATATATATAAGATCGGTAGAG210.13410818059901655No Hit
TATATATATATATATATATATATATATATATATATATATATATATAAGAT200.1277220767609681No Hit
TATATATATATATATATATATATATATATATATATATATAAGATCGGAAG200.1277220767609681No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATA200.1277220767609681No Hit
CTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCACCA180.11494986908487131Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATATATATATAAGATCGGTAGAGCG170.10856376524682292No Hit
TATATATATATATATATATATATATATATATATATATATAAGATCGGTAG170.10856376524682292No Hit
TATATATATATATATATATATATATATATATATATATATATAAGATCGGT160.10217766140877452No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)