FastQCFastQC Report
Fri 4 Aug 2023
HK7N5DMXY_n02_CIVR_Y4_D7_P1_C12_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7N5DMXY_n02_CIVR_Y4_D7_P1_C12_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25440238
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC2479520.9746449699094797No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1485290.5838349468271484No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA910460.35788187201707783No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC807610.3174537911162624No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT655760.25776488411782944No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG653480.25686866608716474No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA650100.25554006216451275No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC630590.247871108752992No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA590990.2323052166414481No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC590130.23196716948953072No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG576410.2265741381821978No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT572310.22496251803933595No Hit
GCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCG554150.21782422004070875No Hit
CTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGC527010.2071560808511304No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG521480.204982359048685No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG521340.20492732811697753No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC513150.20170801861209003No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA502240.19741953671974294No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG498710.19603197108454726No Hit
TTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAG495340.1947072979427315No Hit
CGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCG494350.1943181506399429No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC487450.19160591186293147No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT482590.18969555237651473No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG443890.17448343054023316No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC427960.16822169666808934No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA426960.1678286185844645No Hit
GGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCG417850.16424767724264216No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC392950.15446003296038346No Hit
GGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGC391720.1539765469175249No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA390840.15363063820393502No Hit
CAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGC384920.15130361594887595No Hit
GAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGC374340.14714484982412507No Hit
GAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCC372070.14625256257429667No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC366710.1441456640460675No Hit
CGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGG363120.14273451372585427No Hit
GGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACA362950.14266769045163807No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA361230.14199159614780332No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT354650.13940514235755186No Hit
TGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACC353570.13898061802723702No Hit
CGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCC352670.13862684775197465No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT351350.13810798468158986No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC339620.13349717876067038No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT327430.12870555692128352No Hit
CGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAG324870.1276992770272039No Hit
GGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACC321400.12633529607702568No Hit
TCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAG311250.1223455535282335No Hit
GCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCT305810.12020720875331432No Hit
CGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGAC301580.11854448845958124No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA298490.11732987718118044No Hit
CCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCC297560.11696431456340935No Hit
CGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGC296000.11635111275295458No Hit
CTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGG289290.11371355881183186No Hit
GGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCG285590.11225916990241995No Hit
CACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGG285360.11216876194318623No Hit
CGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCC284430.11180319932541513No Hit
TAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTC282390.11100132003482044No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC281310.1105767957045056No Hit
CGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTG280700.11033701807349444No Hit
TCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTG279160.10973167782471217No Hit
GCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCA277490.10907523742505869No Hit
TGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTG275960.10847382795711266No Hit
CAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGA274420.10786848770833041No Hit
GGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCT274340.10783704146164041No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC274260.10780559521495042No Hit
GCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCA273920.10767194866651798No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA272650.10717273950031443No Hit
GCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGC272520.1071216393494432No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA272490.10710984700693445No Hit
GTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCT271410.1066853226766196No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT264610.10401239170797066No Hit
GCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCA262290.103100450553961No Hit
CGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCG258700.10168930023374781No Hit
GCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTG258370.10155958446615161No Hit
CGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCA254530.10005016462503222No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)