FastQCFastQC Report
Mon 1 May 2023
HK7N5DMXY_n01_CIVR_Y4_D7_P2_H2O.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7N5DMXY_n01_CIVR_Y4_D7_P2_H2O.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181578
Sequences flagged as poor quality0
Sequence length151
%GC76

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCTCGTAT8926649.161242000682904TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCGCGTAT3979621.916752029430878TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCTCGGAT35141.9352564738018923TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCTCGTTT29471.6229939750410292TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCGCGGAT22811.2562094526869996TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCCCTCGGGTATCTCGTAT19731.086585379286037TruSeq Adapter, Index 16 (97% over 35bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCTCGTA14730.8112216237649936TruSeq Adapter, Index 16 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCGCGTTT13610.7495401425282798TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCCCTCGGGTATCGCGTAT9720.5353071407329082TruSeq Adapter, Index 16 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCTCGTATG9140.5033649450924671TruSeq Adapter, Index 23 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCTCGTA8940.4923503948716254TruSeq Adapter, Index 23 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCGCGTA7000.38550925772946065TruSeq Adapter, Index 16 (97% over 35bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGG5670.3122624987608631TruSeq Adapter, Index 23 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCGCGTATG5010.2759144830320854TruSeq Adapter, Index 23 (97% over 36bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGG4670.2571897476566545TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCGCGTA4590.25278392756831775TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCTGGTAT4540.25003029001310734TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCTCGTAA3560.19605899393098283TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTCTCTCGTAT3000.165218253312626TruSeq Adapter, Index 23 (97% over 37bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2490.13713115024947956Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTC2400.13217460265010078TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTCTCGCGTAT2350.12942096509489034TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCGGGTAT2220.12226150745134323TruSeq Adapter, Index 23 (97% over 37bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCTCGTAT2160.1189571423850907TruSeq Adapter, Index 16 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGGTATCTCGGTT2010.1106962297194594TruSeq Adapter, Index 23 (97% over 37bp)
GCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTC1860.10243531705382809TruSeq Adapter, Index 23 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)