FastQCFastQC Report
Mon 1 May 2023
HK7N5DMXY_n01_CIVR_Y4_D7_P2_G10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7N5DMXY_n01_CIVR_Y4_D7_P2_G10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171574
Sequences flagged as poor quality0
Sequence length151
%GC74

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTTATCTCGTAT6580438.35313042768718TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTTATCGCGTAT3926722.88633475934582TruSeq Adapter, Index 15 (97% over 37bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2118612.348024758996118Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTTATCTCGTA22431.3073076340238032TruSeq Adapter, Index 19 (97% over 36bp)
ATGGGCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTT20831.2140534113560328TruSeq Adapter, Index 15 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTTATCGCGTA14080.8206371594763775TruSeq Adapter, Index 19 (97% over 36bp)
ATGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTTATC14030.8177229650180097TruSeq Adapter, Index 15 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTTATCTCGTA11430.6661848531828831TruSeq Adapter, Index 15 (97% over 37bp)
ATGGGCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTT9030.5263035191812279TruSeq Adapter, Index 15 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTTATCGCGTA8610.5018242857309383TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTTATCTGGTAT7250.4225581964633336TruSeq Adapter, Index 15 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTTATCTCGTATG6350.3701026962127129TruSeq Adapter, Index 15 (97% over 36bp)
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGAGATCGGAA6270.36543998507932435ABI Solid3 Adapter B (100% over 41bp)
ATGGGCAGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTG5970.34795481832911745TruSeq Adapter, Index 15 (97% over 37bp)
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATAGATCGGAA5870.34212642941238186ABI Solid3 Adapter B (100% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGGTGTTTATCTCGTAT5430.31648151817874504TruSeq Adapter, Index 11 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTTATCGCGTATG4860.2832597013533519TruSeq Adapter, Index 15 (97% over 36bp)
ATGGGCAGTTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4560.265774534603145Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTTAT4510.2628603401447772TruSeq Adapter, Index 15 (97% over 37bp)
GCCTTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGG4340.2529520789863266No Hit
ATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGGCCTTA4340.2529520789863266No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTTATCGGGTAT4280.24945504563628523TruSeq Adapter, Index 15 (97% over 37bp)
ATGGGCAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGT3510.20457645097742083TruSeq Adapter, Index 15 (97% over 37bp)
ATGGGCAGTCCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3500.20399361208574723Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTT3350.1952510287106438TruSeq Adapter, Index 15 (97% over 37bp)
ATGGGCAGTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGT3330.19408535092729667TruSeq Adapter, Index 15 (97% over 37bp)
ATGGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTTA2930.17077179526035413TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGGTTTATCTCGTAT2870.16727476191031276TruSeq Adapter, Index 20 (97% over 37bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTT2700.15736650075186218TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGGTGTTTATCGCGTAT2550.14862391737675873TruSeq Adapter, Index 11 (97% over 37bp)
ATGGGCAGTCCGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2520.14687540070173802Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGG2430.14162985067667594No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTGTATCTCGTAT2170.1264760394931633TruSeq Adapter, Index 15 (97% over 37bp)
CCTCTCTATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTC2140.12472752281814262Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTTATCTCGGAT2030.11831629500973341TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTTATCTCGTTT2000.11656777833471271TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGTTTATCGCGGAT1960.11423642276801847TruSeq Adapter, Index 15 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)