FastQCFastQC Report
Fri 4 Aug 2023
HK7N5DMXY_n01_CIVR_Y4_D7_P2_F2_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7N5DMXY_n01_CIVR_Y4_D7_P2_F2_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11385982
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC942750.8279918236301445No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA564270.4955830775070609No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC391020.3434222889163183No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG286450.25158128653286116No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCCGAAATCTCGTTT249040.21872509547266103TruSeq Adapter, Index 18 (97% over 37bp)
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC244190.21446547166507024No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCCGAAATCTCGGTT233370.20496255834586774TruSeq Adapter, Index 18 (97% over 37bp)
ACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA207030.18182884884237477No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCCGAAATCTCGTAT198710.17452161789821904TruSeq Adapter, Index 18 (97% over 37bp)
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT183570.1612245654349357No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT180520.15854583293737862No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG175500.15413690272828467No Hit
GGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCA164530.14450224846657933No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC158580.13927652441396798No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCCGAAATCGCGGTT153470.13478854963937234TruSeq Adapter, Index 18 (97% over 37bp)
CCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGAC143450.12598825468018482No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG140060.12301090937962136No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG139760.12274742749461576No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC138170.121350973504086No Hit
GGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCC136000.11944512120254538No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT134170.11783788170401112No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCCGAAATCGCGTTT130750.1148341882149471TruSeq Adapter, Index 18 (97% over 37bp)
CACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGG123290.10828227200780749No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCCGAAATCTCGGAT122770.10782557007379776TruSeq Adapter, Index 18 (97% over 37bp)
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC121300.10653450883727025No Hit
CGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGA120880.10616563419826239No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCCGAAATCGCGTAT116990.10274915242268957TruSeq Adapter, Index 18 (97% over 37bp)
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCT115840.10173913853016806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)