FastQCFastQC Report
Mon 1 May 2023
HK7N5DMXY_n01_CIVR_Y4_D7_P2_F10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7N5DMXY_n01_CIVR_Y4_D7_P2_F10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27553078
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC2123710.7707705106485744No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA1564550.5678312963800269No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC1352200.49076186696818414No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT1165350.42294730193120345No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA1151300.4178480531285833No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC1114350.40443757318147905No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC986810.35814873387285445No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG983000.35676594825449265No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA895080.32485662763339906No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA840590.3050802527398209No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT782250.2839065747935675No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG721400.26182192784414143No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC700530.2542474564910679No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC627060.22758255901572957No Hit
CTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGC617280.2240330463260765No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG612960.2224651634202175No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA593030.21523185177351145No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC591170.21455679107793327No Hit
CTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGAT572360.20772996759200552No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG551770.20025711827912654No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC532690.1933323021115826No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC522580.1896630205888431No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGAC521760.18936541318541616No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC519280.18846533225797857No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT502130.18224098229606145No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA486730.17665177008536034No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA477200.17319299136016672No Hit
CGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCG472540.17150171026264288No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC462820.1679739737244601No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC462570.1678832397600007No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC458780.16650771285879568No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT451120.16372762418775863No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT448680.16284206069463456No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT432500.15696975851482No Hit
TGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACC407220.1477947400286821No Hit
GGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGC406270.14744995096373625No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC391800.14219826910082423No Hit
CTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTT384510.13955246669718716No Hit
GTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAA383640.1392367125008683No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT383250.1390951675163116No Hit
GCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCG381240.13836566644205775No Hit
GAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCA381160.13833663157343074No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG377700.1370808735053122No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA376770.1367433431575231No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT367750.13346966171982674No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA358410.1300798408076223No Hit
CAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATG354900.12880593594661183No Hit
CAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCG354190.12854825148754706No Hit
GGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGG337910.12263965572194874No Hit
CAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACC333300.12096652141731679No Hit
GTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCA333300.12096652141731679No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC322420.11701777928404224No Hit
CAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGC320380.11627739013405326No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG318320.11552974226690753No Hit
CGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGAC316870.11500348527304283No Hit
CTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGA314660.11420139702722143No Hit
CGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGG312500.1134174555742919No Hit
GGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACA310690.11276054167160562No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT309320.11226331954636791No Hit
GCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTC307920.1117552093453951No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG306070.11108377800839529No Hit
CCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAG304250.11042323474713062No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC299380.10865573711946085No Hit
CCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTAT299370.10865210776088248No Hit
GGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCT298470.10832546548882852No Hit
CAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCC296290.10753426531874224No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT295000.10706607806213156No Hit
CGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACC290240.10533850337882396No Hit
CGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGA289020.10489572163226192No Hit
GGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGC286340.10392305353325679No Hit
GGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACA282250.10243864587470046No Hit
TTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAG280220.10170188608328987No Hit
CTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTG278340.10101956667055492No Hit
CGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAG276240.10025740136909568No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)