FastQCFastQC Report
Mon 1 May 2023
HK7N5DMXY_n01_CIVR_Y4_D7_P2_D6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7N5DMXY_n01_CIVR_Y4_D7_P2_D6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126784
Sequences flagged as poor quality0
Sequence length151
%GC75

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGGTT3311026.11528268551237TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCGCGGTT1537512.12692453306411TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGGGT119849.452296819787986TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCGCGGGT81066.393551236749116TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGTTT73235.77596542150429TruSeq Adapter, Index 22 (97% over 37bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC58264.595217062089853Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCGCGTTT29112.2960310449268047TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGTGT25792.034168349318526TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGGAT21361.6847551741544675TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCGCGTGT14051.1081839979808177TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCGCGGAT11070.8731385663806159TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGTAT8780.6925164058556285TruSeq Adapter, Index 22 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGGT7880.6215295305401313TruSeq Adapter, Index 22 (97% over 37bp)
ATGGGCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAA7360.5805148914689551TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGGTG7130.5623738011105502TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGGT6550.5166267036850075TruSeq Adapter, Index 22 (97% over 37bp)
ATGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATC5600.441696113074205TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGGGG4660.3675542655224634TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCGCGTAT3660.28867995961635534TruSeq Adapter, Index 22 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCGCGGT3570.28158127208480566TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCGCGGTG3360.26501766784452296TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTGGGTT3350.2642289247854619TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCGCGGT3160.24924280666330134TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCGCGGGG3010.23741166077738515TruSeq Adapter, Index 22 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGGTTG2710.21374936900555275TruSeq Adapter, Index 22 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGGG2610.20586193841494196TruSeq Adapter, Index 22 (97% over 37bp)
ATGGGCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAA2440.19245330641090358TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGGG2270.17904467440686522TruSeq Adapter, Index 22 (97% over 37bp)
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATAGATCGGAA2110.16642478546188794ABI Solid3 Adapter B (100% over 41bp)
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGAGATCGGAA2080.1640585562847047ABI Solid3 Adapter B (100% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCGCGGG1840.14512872286723877TruSeq Adapter, Index 22 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGTT1740.13724129227662796TruSeq Adapter, Index 22 (97% over 37bp)
ATGGGCAGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAT1700.13408632004038365TruSeq Adapter, Index 22 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCGCGGG1670.1317200908632004TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCGGGGTT1620.127776375567895TruSeq Adapter, Index 22 (97% over 37bp)
ATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGGCCTTA1610.1269876325088339No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCGCGGTTG1600.12619888944977284TruSeq Adapter, Index 22 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTGGGGT1490.11752271580010097TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGGTA1460.11515648662291772TruSeq Adapter, Index 22 (97% over 37bp)
ATGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAAT1340.10569156991418475TruSeq Adapter, Index 22 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGGGTG1340.10569156991418475TruSeq Adapter, Index 22 (97% over 36bp)
ATGGGCAGTTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1330.10490282685512368Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATAAAATCTCGTT1290.10174785461887935TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)