FastQCFastQC Report
Fri 4 Aug 2023
HK7N5DMXY_n01_CIVR_Y4_D7_P1_H12_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7N5DMXY_n01_CIVR_Y4_D7_P1_H12_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47507
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTAT25385.342370598017134TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTTT25185.300271538931105TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGGTT12112.549098027659082TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGTAT11872.4985791567558464TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGTTT11472.414381038583788TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGGAT9271.951291388637464TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGGTT7041.4818868798282359TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGGAT5161.0861557244195592TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTT3380.7114740985538973TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTA3280.6904245690108826TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTGT2820.593596733113015TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGGT2130.44835497926621337TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGTGT1920.40415096722588256TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGTA1800.3788915317742648TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGTT1680.3536320963226472TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGGGT1500.3157429431452207TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGGT1350.28416864883069864TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGGGT1350.28416864883069864TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGGA1110.23364977792746333TruSeq Adapter, Index 23 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTT1060.22312501315595598TruSeq Adapter, Index 23 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTA800.1683962363441177TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGGA770.1620813774812133TruSeq Adapter, Index 23 (97% over 37bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAAC680.14313680089250005TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAAC610.12840213021238975TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTG510.10735260066937503TruSeq Adapter, Index 23 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGTA490.1031426947607721TruSeq Adapter, Index 23 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGGT480.10103774180647064TruSeq Adapter, Index 23 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)