FastQCFastQC Report
Mon 1 May 2023
HK7N5DMXY_n01_CIVR_Y4_D7_P1_E9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7N5DMXY_n01_CIVR_Y4_D7_P1_E9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15919922
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC1298680.8157577656473443No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT1095630.6882131708936765No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC908140.5704424933740253No Hit
CTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGC724790.4552723311081549No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC706650.4438778029188836No Hit
GGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCG666080.41839400971939433No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC595150.37383977132551277No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT588580.3697128666836433No Hit
CAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGC588470.3696437708677216No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA561970.3529979606684002No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC519510.32632697572261976No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG511410.3212390110956574No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG496710.31200529751339234No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC464660.29187328932893014No Hit
CCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGA454780.2856672287715982No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG452880.284473755587496No Hit
CGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCG439840.2762827606818677No Hit
CGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCG426330.2677965381991193No Hit
CGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGG417240.2620867112288615No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA402790.25301003359187313No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA387440.24336802655188888No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA373190.23441697767112177No Hit
GTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGT371260.2332046601735863No Hit
CAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTAGACCACCA366570.23025866584019694No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT364820.22915941422326064No Hit
GGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGC355330.2231983297405603No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA350800.220352838412148No Hit
GCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTC347770.2184495627553954No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA346650.21774604172055617No Hit
GGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTAGACCA336600.21143319672043617No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC335590.21079877150151868No Hit
GCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGA332390.20878871140197797No Hit
CTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTT326630.20517060322280475No Hit
GCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTG323740.20335526769540707No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT321080.20168440523766384No Hit
TGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCAC314490.19754493772017223No Hit
CGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACA309160.1941969313668748No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC308300.1936567277151232No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT301540.18941047575484352No Hit
CTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAA297190.18667805030703039No Hit
CGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCC291670.18321069663532272No Hit
GCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCT289760.18201094201340937No Hit
GGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCT289290.1817157144362893No Hit
GCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCT288310.18110013353080498No Hit
GCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCG284660.1788074087297664No Hit
GTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAA275690.1731729590132414No Hit
CGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAG275560.17309130032169753No Hit
CTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGG273730.17194179720227273No Hit
TAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTC272700.17129480910773306No Hit
CTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGAT271660.17064153957538233No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG268970.16895183280420595No Hit
CAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATG268950.16893926992858382No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC266050.16711765296337505No Hit
CGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGAC265160.1665586049981903No Hit
GCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGC264950.16642669480415798No Hit
CAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGG264160.16593046121708385No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG263710.16564779651558595No Hit
TTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAG263140.16528975456035525No Hit
GCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTC262320.16477467665984796No Hit
GGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGG260510.16363773641604526No Hit
GTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGC255970.1607859636498219No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA255420.16044048457021337No Hit
CAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGG252570.15865027479405994No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC250000.1570359452766163No Hit
CAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCT249700.15684750214228438No Hit
TCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTG246300.1547118132865224No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG245730.1543537713312917No Hit
GGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGT237390.14911505219686377No Hit
GCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCA235600.1479906748286832No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC231580.14546553682863522No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA228680.14364391986342648No Hit
GGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAGCTTAACGG228490.14352457254501624No Hit
TGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTG225460.14162129688826364No Hit
CTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCA223840.14060370396287117No Hit
CTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAA222510.13976827273399955No Hit
CACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCT222230.13959239247528976No Hit
TGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACC220410.138449170793676No Hit
CGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCC219850.13809741027625638No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG217910.13687881134090985No Hit
GAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCA217040.13633232625134722No Hit
GTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTT214860.13496297280853511No Hit
CAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCC210750.13238130186818753No Hit
GCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTC210340.1321237629179339No Hit
GGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCC209720.1317343137736479No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT209090.1313385831915508No Hit
CTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTT208640.1310559184900529No Hit
GCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCAC207520.13035239745521368No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT206740.12986244530595062No Hit
CGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGA205860.1293096787785769No Hit
CGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCA205860.1293096787785769No Hit
CTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCA205270.12893907394772414No Hit
CCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCC204430.12841143317159467No Hit
GCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCA203770.1279968582760644No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT202550.12723052286311454No Hit
CGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGC202400.12713630129594858No Hit
GTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGG201280.12643278026110932No Hit
CGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGG199500.12531468433073983No Hit
GAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCC199290.12518277413670745No Hit
AAAGGACTCAAAGAACCTCTGGGTCCAAGGGTAGACCACCAGCAGCCTGC198740.12483729505709888No Hit
CCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAG197820.12425940277848095No Hit
GGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACG197010.12375060631578472No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCAC196560.1234679416142868No Hit
CCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTC194340.12207346242023044No Hit
GGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGC192710.12104958805702691No Hit
CCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTAT191180.12008852807193401No Hit
AGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTC190700.11978701905700291No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC190610.11973048611670335No Hit
CTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCC189710.1191651567137075No Hit
CTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC188830.11861239018633382No Hit
GCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAA186910.11740635412660942No Hit
GTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCA185680.11663373727584846No Hit
GCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGG184870.11612494081315224No Hit
TTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAG184750.11604956355941945No Hit
CTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGT183920.11552820422110108No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC182170.11442895260416477No Hit
CCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGG181010.11370030581808127No Hit
GTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCG179980.11305331772354162No Hit
GAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGC179380.11267643145487774No Hit
CTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAA179330.1126450242658224No Hit
GCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCC178280.11198547329566062No Hit
GCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTA176390.11079828154936941No Hit
GTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCT175190.11004450901204163No Hit
CGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACC174260.10946033529561264No Hit
GCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCC174210.1094289281065573No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC173130.10875053282296233No Hit
CTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGG172660.10845530524584228No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA171950.10800932316125669No Hit
GCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTA171070.107456556633883No Hit
TGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTG170080.10683469429058759No Hit
GGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCT167640.10530202346468784No Hit
GTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTC167190.10501935876318991No Hit
CTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAGGA166910.10484347850448011No Hit
CGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCAT166730.10473041262388096No Hit
CTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATT166600.10464875393233711No Hit
CAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCG166590.10464247249452605No Hit
GTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTG164530.10334849630544672No Hit
GGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGG162690.10219271174821082No Hit
CTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGA161280.10130702901685071No Hit
CCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGC160890.10106205294221919No Hit
CGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTG160830.10102436431535279No Hit
GGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACT160830.10102436431535279No Hit
CCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAG160540.10084220261883192No Hit
GCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCA160430.10077310680291021No Hit
GTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGG160390.10074798105166596No Hit
CGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGT160220.10064119660887787No Hit
CATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGG159930.10045903491235697No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)