FastQCFastQC Report
Fri 4 Aug 2023
HK7N5DMXY_n01_CIVR_Y4_D7_P1_D6_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7N5DMXY_n01_CIVR_Y4_D7_P1_D6_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15659
Sequences flagged as poor quality0
Sequence length151
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTTT12057.695255124848329TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTAT11437.299316686889329TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGGTT6634.233986844626093TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGTAT5723.6528513953636885TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGTTT5043.2185963343763966TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGGAT4803.0653298422632353TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGGTT3572.2798390701832814TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGGAT2811.7944951784916021TruSeq Adapter, Index 22 (97% over 38bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2101.3410818059901655Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTT1661.0600932371160354TruSeq Adapter, Index 22 (97% over 38bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1631.0409349256018903Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTGT1500.957915575707261TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTA1320.8429657066223897TruSeq Adapter, Index 22 (97% over 38bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1220.7791046682419056Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC1140.7280158375375183Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGGT1060.6769270068331311TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGTT1000.6386103838048407TruSeq Adapter, Index 22 (97% over 38bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT960.613065968452647Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGTGT940.6002937607765502TruSeq Adapter, Index 22 (97% over 38bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA840.5364327223960662Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCACA740.47257168401558214No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC710.4534133725014369No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGGGT710.4534133725014369TruSeq Adapter, Index 22 (97% over 38bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA700.44702726866338843Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGGT670.4278689571492433TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGTA660.42148285331119484TruSeq Adapter, Index 22 (97% over 38bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG640.40871064563509807No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG630.4023245417970496Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGGGT620.3959384379590012TruSeq Adapter, Index 22 (97% over 38bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTT600.3831662302829044TruSeq Adapter, Index 12 (97% over 36bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG590.37678012644485603Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGGA550.3512357110926624TruSeq Adapter, Index 22 (97% over 38bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT540.344849607254614Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC540.344849607254614Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCA520.3320773995785171No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTA510.32569129574046873TruSeq Adapter, Index 12 (97% over 36bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC470.3001468803882751No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGGA440.28098856887412993TruSeq Adapter, Index 22 (97% over 38bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG410.26183025735998466Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATATATATATATATATATATATAAGATCGGAAGAG390.24905804968388787No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGTA370.23628584200779107TruSeq Adapter, Index 12 (97% over 36bp)
TATATATATATATATATATATATATATATATATATATATAAGATCGGAAG350.22351363433169422No Hit
TATATATATATATATATATATATATATATATATATATATATATAAGATCG340.21712753049364583No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT320.20435532281754903TruSeq Adapter, Index 1 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTG270.172424803627307TruSeq Adapter, Index 22 (97% over 38bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGGT260.16603869978925856TruSeq Adapter, Index 12 (97% over 36bp)
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCACA250.15965259595121017No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTAA230.14688038827511335TruSeq Adapter, Index 22 (97% over 38bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAG220.14049428443706496TruSeq Adapter, Index 22 (97% over 38bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG210.13410818059901655No Hit
TATATATATATATATATATATATATATATATATATATATATAAGATCGGA200.1277220767609681No Hit
TATATATATATATATATATATATATATATATATATATATATATATAAGAT200.1277220767609681No Hit
ATATATATATATATATATATATATATATATATATATAGATCGGAAGAGCA190.12133597292291973No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGGA190.12133597292291973TruSeq Adapter, Index 12 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTTA180.11494986908487131TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGGG180.11494986908487131TruSeq Adapter, Index 22 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTTTG170.10856376524682292TruSeq Adapter, Index 22 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGTT170.10856376524682292TruSeq Adapter, Index 12 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGGG170.10856376524682292TruSeq Adapter, Index 22 (97% over 38bp)
TATATATATATATATATATATATATATATATATATATATATATATATATA170.10856376524682292No Hit
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAG160.10217766140877452TruSeq Adapter, Index 22 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)