FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11803523
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6103775.171142547864735No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN767520.6502465407997257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAC21350.028.4134543
GGATAGC27200.027.3521565
GACAGTA30700.027.2374297
CGAAAGC18800.027.2134783
TTGGACA32800.026.7193154
TATACTG33700.026.1888085
GCAGGTA23900.024.4822222
TATGGAT34150.023.9875562
ACTGATC28700.023.8036678
ATCCAAT52350.022.9178527
TGGATAG33100.022.5055734
GGACAGT48750.021.499726
GTATGGA41150.021.1840781
GTGATCG5353.6379788E-1220.3738889
AACAAGG60800.020.0915017
TCCAATG60900.020.048878
CCTTATA35350.019.8628032
ATACTGG40500.018.6906016
ACAAGGC37200.018.5600288
AACGAGG27700.018.262096145
Produced by FastQC (version 0.11.5)