FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_pDZ_amp_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_pDZ_amp_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG80.2789400278940028No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT70.24407252440725244No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA70.24407252440725244No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA60.20920502092050208No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT60.20920502092050208No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG60.20920502092050208No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA60.20920502092050208No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG60.20920502092050208No Hit
GAATTAGCTCATCCTTCAGTTTTGGTGGATTCACATTTAAGAGAACAAGC60.20920502092050208No Hit
AGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAAGA50.17433751743375175No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT50.17433751743375175No Hit
CCTATCGACAATGTGATGGGAATGATTGGGATATTGCCAGACATGACTCC50.17433751743375175No Hit
GTAAATGAGGAGAACTGCATTCTACTTTGCTTTGGTGGAGCGGCTGCGAA50.17433751743375175No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT50.17433751743375175No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA50.17433751743375175No Hit
ATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTTTGGTG50.17433751743375175No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA50.17433751743375175No Hit
GTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGAT50.17433751743375175No Hit
CTATGTTCCTAGCAGCAATAATCAAGCTTTGATCAACATCATCATTCCTC40.1394700139470014No Hit
GATTAATGGTCCTGAATCAGTGTTGGTCAATACCTATCAATGGATCATCA40.1394700139470014No Hit
CATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAATGTACA40.1394700139470014No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC40.1394700139470014No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT40.1394700139470014No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG40.1394700139470014No Hit
CCACCAATCTGTGTGCTGTGGCACATCTCCAATAAAGATGCTAGTGGATC40.1394700139470014No Hit
GATAATAAAACTTCTTCCCTTCGCAGCCGCTCCACCAAAGCAAAGTAGAA40.1394700139470014No Hit
TTCTCACTCCTCTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAAT40.1394700139470014No Hit
ATGCTCACCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTT40.1394700139470014No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT40.1394700139470014No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT40.1394700139470014No Hit
TTTCAGAAGGATGCGAAAGTGCTTTTTCAAAATTGGGGAGTTGAACCTAT40.1394700139470014No Hit
GAATAGGAATGGACCAATAACAAATACAGTTCATTATCCAAAAATCTACA40.1394700139470014No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT40.1394700139470014No Hit
GATCAGGAATGAGAATACTTGTAAGGGGCAATTCTCCTGTATTCAACTAT40.1394700139470014No Hit
ATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAGTGCTT40.1394700139470014No Hit
GTACTAAAGACTGAAATGGTTCAAATTCCATTTTATTGTATAGCATTGTA40.1394700139470014No Hit
GGATACTAACATCGGAATCGCAACTAACGATAACCAAAGAGAAGAAAGAA40.1394700139470014No Hit
CTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACTGACAATAACT40.1394700139470014No Hit
GTGTAGATGAGTACTCCAGCACGGAGAGGGTAGTGGTGAGCATTGACCGT40.1394700139470014No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC40.1394700139470014No Hit
GTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGATGT40.1394700139470014No Hit
GTATCAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTAC40.1394700139470014No Hit
GTTCCATGCTTTAGCCTTTCGACTCTTTCAAAATAAGTTTTGTAGATTTT30.10460251046025104No Hit
TCTCTGTTCCCTGTGTTTCACTGACCTCCTCGGGAGACAGTAGTACATTT30.10460251046025104No Hit
GAATTAGGATGGTAGACATCCTTAGGCAGAACCCAACAGAAGAGCAAGCC30.10460251046025104No Hit
GCAATAATTGTGGCCATGGTATTTTCACAAGAGGATTGTATGATAAAAGC30.10460251046025104No Hit
GGAATGGACCAATAACAAATACAGTTCATTATCCAAAAATCTACAAAACT30.10460251046025104No Hit
ATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGGGATGTGCTTG30.10460251046025104No Hit
GAGGAAGAGGTGCTTACGGGCAATCTTCAAACATTGAAGATAAGAGTGCA30.10460251046025104No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT30.10460251046025104No Hit
AATGTACACACTGCTTGTTCCACCAGCCACTGGGAGGAATCTCGTTTTGC30.10460251046025104No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA30.10460251046025104No Hit
GTTGGAGAGAGAACTGGTCCGCAAAACGAGATTCCTCCCAGTGGCTGGTG30.10460251046025104No Hit
GTTCCTAGCAGCAATAATCAAGCTTTGATCAACATCATCATTCCTCACTT30.10460251046025104No Hit
GTTATTGTCAGTTTCTCTGTTCCCTGTGTTTCACTGACCTCCTCGGGAGA30.10460251046025104No Hit
GTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGAAAAGCAACCA30.10460251046025104No Hit
GTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGA30.10460251046025104No Hit
GTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTCACC30.10460251046025104No Hit
AGTCAAGAGAGAGGAAGAGGTGCTTACGGGCAATCTTCAAACATTGAAGA30.10460251046025104No Hit
ATGTTGGAGAGAGAACTGGTCCGCAAAACGAGATTCCTCCCAGTGGCTGG30.10460251046025104No Hit
GCATACATGTTGGAGAGAGAACTGGTCCGCAAAACGAGATTCCTCCCAGT30.10460251046025104No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA30.10460251046025104No Hit
GTGGAATAGGAATGGACCAATAACAAATACAGTTCATTATCCAAAAATCT30.10460251046025104No Hit
CATCTACACCCATTTTGCTGATTCTCACTCCTCTCATTGACATCTCGATG30.10460251046025104No Hit
ACACTGATTCAGGACCATTAATCTCCCACATCATTGACGATGAGTAAGTT30.10460251046025104No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC30.10460251046025104No Hit
ATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAGTCGAA30.10460251046025104No Hit
GTGATGGGAATGATTGGGATATTGCCAGACATGACTCCAAGCATCGAGAT30.10460251046025104No Hit
CTCCTGGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAAC30.10460251046025104No Hit
GAGTGGGAGAGACGAACAGTCGATTGCCGAAGCAATAATTGTGGCCATGG30.10460251046025104No Hit
CTTTAGTACCTAAGGCCATTAGAGGCCAATACAGTGGGTTTGTAAGAACT30.10460251046025104No Hit
GACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGATGAGT30.10460251046025104No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC30.10460251046025104No Hit
GAAGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAA30.10460251046025104No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT30.10460251046025104No Hit
GATTGGGATATTGCCAGACATGACTCCAAGCATCGAGATGTCAATGAGAG30.10460251046025104No Hit
GAGAGGAAGAGGTGCTTACGGGCAATCTTCAAACATTGAAGATAAGAGTG30.10460251046025104No Hit
GATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGC30.10460251046025104No Hit
GCTGATACTGCAGCTCTTCTCACTATGTTCCTAGCAGCAATAATCAAGCT30.10460251046025104No Hit
GCTGATAGTGAGTGGGAGAGACGAACAGTCGATTGCCGAAGCAATAATTG30.10460251046025104No Hit
TAATAAAACTTCTTCCCTTCGCAGCCGCTCCACCAAAGCAAAGTAGAATG30.10460251046025104No Hit
CATACAATCCTCTTGTGAAAATACCATGGCCACAATTATTGCTTCGGCAA30.10460251046025104No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT30.10460251046025104No Hit
CTCTTAAATGTGAATCCACCAAAACTGAAGGATGAGCTAATTCTCAGTCC30.10460251046025104No Hit
GTATGGCTGTTGCTCTTCTCCCAACCATTGTGAACTCTTCATATCCCTCA30.10460251046025104No Hit
GGAACATAGTGAGAAGAGCTGCAGTATCAGCAGATCCACTAGCATCTTTA30.10460251046025104No Hit
GCTAATTCTCAGTCCCATTGCAGCCTTGCATATATCCACGGCTTGCTCTT30.10460251046025104No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT30.10460251046025104No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA30.10460251046025104No Hit
ATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTG30.10460251046025104No Hit
CCATTAATCTCCCACATCATTGACGATGAGTAAGTTATTGTCAGTTTCTC30.10460251046025104No Hit
GTGTGCTGTGGCACATCTCCAATAAAGATGCTAGTGGATCTGCTGATACT30.10460251046025104No Hit
GATTGATTCAGCTGATAGTGAGTGGGAGAGACGAACAGTCGATTGCCGAA30.10460251046025104No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT30.10460251046025104No Hit
TATCTCTTGTCTTCTTTGCCCAGAATGAGGAATCCCCTCAGAACAGCGGA30.10460251046025104No Hit
CCTTGGCACTGAGATCTGCATGACCAGGATTTATGTCAACTCTCCGACGT30.10460251046025104No Hit
GCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCA30.10460251046025104No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC30.10460251046025104No Hit
CTTCTGAAAATGTCTTAAAAGTTGATGCATAGGATTCAATCGCTGATTCG30.10460251046025104No Hit
GCTAGGAACATAGTGAGAAGAGCTGCAGTATCAGCAGATCCACTAGCATC30.10460251046025104No Hit
CAACTAACGATAACCAAAGAGAAGAAAGAAGAACTCCAGGATTGCAAAAT30.10460251046025104No Hit
CTGATGATCCATTGATAGGTATTGACCAACACTGATTCAGGACCATTAAT30.10460251046025104No Hit
ATCCTCTTGTGAAAATACCATGGCCACAATTATTGCTTCGGCAATCGACT30.10460251046025104No Hit
CAATAATTGTGGCCATGGTATTTTCACAAGAGGATTGTATGATAAAAGCA30.10460251046025104No Hit
GCTTACGGGCAATCTTCAAACATTGAAGATAAGAGTGCATGAGGGATATG30.10460251046025104No Hit
GCATTCTACTTTGCTTTGGTGGAGCGGCTGCGAAGGGAAGAAGTTTTATT30.10460251046025104No Hit
GGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGGGATGT30.10460251046025104No Hit
GTATCACCTCTGGCTGTGACATGGTGGAATAGGAATGGACCAATAACAAA30.10460251046025104No Hit
TCAATGATGTGGGAGATTAATGGTCCTGAATCAGTGTTGGTCAATACCTA30.10460251046025104No Hit
AGAGAGAACTGGTCCGCAAAACGAGATTCCTCCCAGTGGCTGGTGGAACA30.10460251046025104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACT100.006726674145.04
TCGACAC100.006726674145.03
ACTGACC100.006726674145.0145
TTCGACA100.006726674145.02
ACACTAA100.006726674145.06
GACACTA100.006726674145.05
ATTCGAC100.006726674145.01
CACTAAT100.006726674145.07