FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_Negative_Control_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_Negative_Control_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGAGATTGCCGAGGAGACCAGTGTTATTATGGAGTGGAGGAAGGA50.2002402883460152No Hit
GCCTATAGCATCAGGCCACCACAGCTCTGAACTGTGCCTGGGACCATCTG50.2002402883460152No Hit
GTCCAGGCTAACAGCAGCAGAAGTGGAATCAGTCTCCAGACCATGTCTTT40.16019223067681218No Hit
GTAAAAGGCAGTGCCCCAGCTCTGCTAAGTCCTCCCAATCAGCACTGAAG30.12014417300760913No Hit
GTGTCACCGGGGTCCAGGTGGCTGGTGGGACGTCCCGGTGTAGGAGCCGG30.12014417300760913No Hit
CTACACAGCAGATTTAAAAATACAGAGTGCTAAGGTTGGCACAGGGGCGA30.12014417300760913No Hit
GTGCTCATCTTGAGCCTAAAGGAGTTTGGGCAGCTGTCAAATGCAGCAGC30.12014417300760913No Hit
GTCCAGACCCATGCCCTGCTCCCCTGAAAAGGGCCAGGGACACAGAGTGC30.12014417300760913No Hit
TCACGGATCTCTGCACTTGTTTTCTGAAGGGTAAGGTAGGAATCTGGTGC30.12014417300760913No Hit
GCACATGGACGCTGTCCAGGCTAACAGCAGCAGAAGTGGAATCAGTCTCC30.12014417300760913No Hit
GTGTAGGAGCCGGCCCTGTGGCCTTAGGTGGGCTCAGAGAGAGGGGCTGG30.12014417300760913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA953.087345E-513.736842135-139
Produced by FastQC (version 0.11.5)