FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_Negative-Control-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_Negative-Control-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTATTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA10.9615384615384616No Hit
ATGCGGCCCATTAGGTTGATGTGTGGATGTTGAACCACCATTGCAGCCCT10.9615384615384616No Hit
GGAATCCCGACATGGGACTCGATCCCAGGACTCCGGGATCATGACCTGAG10.9615384615384616No Hit
CTTAATAGAAGGGAAGATACACAACAGGTTGCTGGAAACATAGGAAACCA10.9615384615384616No Hit
ATCTAGACAAATCAATTCAAAGAAACTGACACAGCAGACACGCCTCCAGC10.9615384615384616No Hit
GTGCTCCCCCGCGTTTTTTTTTTTTTTTAAAAGTTTTTTTGGCATTTTTA10.9615384615384616No Hit
GTTAGGAATCCTAGTAATAATTATGACCTGCGTGTACAATTTCTTAATGA10.9615384615384616No Hit
ACGGTGTACTATGTACCCATTCCTTTAGGTAGAGCCTTTAATACCCACTC10.9615384615384616No Hit
TCTATTAGGTGACTGGCTGGGTTTTCAATATTTTCTAAGGATCATTTAAA10.9615384615384616No Hit
CCATGGGGCCGGAGTTGGTGGGGACAGCAACGCGCGCAGGGGCAAAAAAA10.9615384615384616No Hit
TGAGTAACCTGACCAAAATTAGTACTGTTAAAAAAGCAGAAAAACTTTAC10.9615384615384616No Hit
CTGGTAGTTGGTTGCTGGGGTTTGACAGGTTTTGCAAGTTCGAACAGCGG10.9615384615384616No Hit
TAATTAAAGAGGATGTATATTTCCTGAAAAATCCCCAACACCCATCATCT10.9615384615384616No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.9615384615384616No Hit
AGGAACGACAATATGGATGCCCTGGGGACAGTTAGGGGAGGTGAGCGTGG10.9615384615384616No Hit
AGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGG10.9615384615384616No Hit
GGTATATGGGGGGCGGGGGGGGCCTCGGGGGCGCGGGGGGTGGGGCAGGG10.9615384615384616No Hit
CGCCTTGAGATCTCTCGTTCATCCAAATATTAATTACTGGCCTTCAGAGT10.9615384615384616No Hit
AGTCTGGACTGGCCACAGCGGGCCCTCGGTTTCCACGCCGACTTTCCCAT10.9615384615384616No Hit
GCAGGACTCAATTCCAGGACCCTAAATTCATGACTTGAGCCAAAGGCCAA10.9615384615384616No Hit
CGATTAGCACTGTGGCGTGCGTCTTGGTTGCCGCTTTGCATACCTGGGTG10.9615384615384616No Hit
GCTTGTTACGAGGAAAAGGAATGCTGGCCAGACGCATATGGGAGTGGGCT10.9615384615384616No Hit
GTAGAGAAACCCACAATAATAGTTGGATAATTCACTATCCCATTCTCAAA10.9615384615384616No Hit
GCGTTGCTCTTCGCTGGTGCCTTTGTGCTGGGGTCCGGGCCGCTCGAAGA10.9615384615384616No Hit
CCCTGGCTGAGCAGGGAGCTCAGTGTGGAACTTGATAACAGGACCCTGGG10.9615384615384616No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG10.9615384615384616No Hit
GGAACAGGCAATAAAGGTAAAAAACATCAAATTTAAACAAAAATTTACTG10.9615384615384616No Hit
TAATTACACTAGTACTCTGTTGGCTTTTTTTTTTTTTAAAACCAATTTCC10.9615384615384616No Hit
AGAGAGGGAAGCATAAAAAAAAAAGATAGATGCCCTAAAATCAGGGGGCG10.9615384615384616No Hit
CCGTTGGACAGGGGACTAGGCCAGTGCCTTCCCCAGCCATGAGGAGACGC10.9615384615384616No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTAGGGTC10.9615384615384616No Hit
CACATAATCAAAAGAAAAAACATAATTATATATAAAATTAGTATATTAAA10.9615384615384616No Hit
TTTCTGCTCAGTGGAGAGCCTGCTTCCTCCTCTCTCTCTCTCTCTCTCTC10.9615384615384616No Hit
GCTTTTGTGGGGGGGGGGGGGGCGACCCCCCCCCCCCGGGGGGGGGGGGC10.9615384615384616No Hit
TTACTATTCTTTCCTCCCTTATCTACTGAGGGGAGAAGAGCCAACGAAAG10.9615384615384616No Hit
AAAAAGACAAAAACCACTTTGTATCAAATGTCCATAAAAAAGAAAAACAA10.9615384615384616No Hit
GAAAGTTAATGTGAAAAATTACCTAAACCTCTATAGAACACAGTGACAAA10.9615384615384616No Hit
GGGCACACGTGGGCTTGCCACCAAGCACCCGGCCCTGAACTCGCAGAACC10.9615384615384616No Hit
TTATATTATCCGAGTGGTATTGCTTGCACAGAAGAAACCTACTGGATTTT10.9615384615384616No Hit
ACGTGGTGTTTTTATGATATATGTTATTCACTTTTCATCAAACTGTATGG10.9615384615384616No Hit
GTTCATCACAGATACTAGTATGTAATTTTCTTGGGAACCATGGGAGGTTT10.9615384615384616No Hit
CCTCTGCGCTCTTGACTGACATCTCTCCACCCAGAGAGGGTTATAGAAAA10.9615384615384616No Hit
GCATTTTCCGTTCTTTCCAGGACCTCCGCAAGCCCTGCTCTGGAAGTCAG10.9615384615384616No Hit
GACAAACCCAGGAAGCTAAATGCCCAAAACAAAACTGAACAAATAAACTA10.9615384615384616No Hit
AGCAGAGAACAAGAAATAAACATAGCAAAGGATAAAATCGTTACAAAAGA10.9615384615384616No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA10.9615384615384616No Hit
CATTATAACCATCCCCGTGATCCCATCCCCATCGACCCCCACGGGCTTCA10.9615384615384616No Hit
CGTTGATACCAACCTTGACGGTCATTAAGGGATCTCCTCCAAATGAGGTT10.9615384615384616No Hit
CTTCAAGACGCTCATATTACTCCTGAACCCTTCTCAGTATCCAAGTGTTT10.9615384615384616No Hit
ATCCCGGAAAGTGAGGCATGTGTTCGCGTCCATGTGAGGCCAGTACAGGA10.9615384615384616No Hit
CCTCTCTGCAGGACATAAGCTGTATGAAGGCAGGGACCTTGGCTATCTGG10.9615384615384616No Hit
CCCAGAGCTTACTCATCTTACAATGAATTGTTCAGCTTTTAAAAGTGGTT10.9615384615384616No Hit
TAATAAAAATCACCTCTATCATCAGATGGAACGCCGCTCACCGCGTATTT10.9615384615384616No Hit
CCCCTAACAGTTCCTTTCGGGGCATCACCCATTCCCTACATTGTTAGTGC10.9615384615384616No Hit
TCATACATATTCACAAGTGAAGATCCCCGCACATACAGCTGCCTCCAACC10.9615384615384616No Hit
TCTATTAATTCCCCTCACCCATGGGGCTCCCGCCACCTTCAAAGTGAGCT10.9615384615384616No Hit
CCTCTTTCTATACCCCTTTATCAATTAGTGACTCTTCACATAATTCTGCC10.9615384615384616No Hit
AGCTTACACTTTCATTTGAGTGGAGCACAATTTCTAATAGCTTCCTGAGA10.9615384615384616No Hit
GTGTTGGTAATGAAACGGAAACGGGACTCTAGCATACTTACTGACAGCCA10.9615384615384616No Hit
TTCAGAGCCACACAAAGAGAGTACAGATTAAACAAAAAAAAAAAATTTAA10.9615384615384616No Hit
GCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC10.9615384615384616No Hit
GGACAACGGCATTCATCAAGAAACGTTGGAAGTAAAAGCCATCAGCAAGG10.9615384615384616No Hit
GGTTTACAGTCATCAAAATCTGTTGTAGTTGGTCTTGTGTTAGACACACC10.9615384615384616No Hit
TCAGCCAGCTTGCGCCGCATCTGGATCGCACCGTAAACGACGACCTCAAG10.9615384615384616No Hit
CTCACAGCCACCGTCCAGGGGAGTTTCAAAGAAATCCACTGACCACAAAT10.9615384615384616No Hit
GTGCCCCAAATGTTCCCTACTTTAGTGAGGGGTGGGGGGGGGGGGGGGGG10.9615384615384616No Hit
AATGGGGTAAGAAAAAACCCGGAGGCTCGATCGCGGGAGGGCAGGGTGCT10.9615384615384616No Hit
TGAAGGAGTTCTTGGGGAGCCCCCGGTCTGACAGCCTGTTGTAGTTCAGG10.9615384615384616No Hit
ACCCTTCCCTCACATGGAGTTCCTTTAGGTGGCCAGTCAGGTGCTGTGTC10.9615384615384616No Hit
AAGTAGCCGTTCGGGATGGTCTCGATCCTGTTGCTGTCCAGGTAGAGCTG10.9615384615384616No Hit
GTATAAACAAGGTCGTTTATAAACAATTCAAAACTAATTCAAGGCCATCC10.9615384615384616No Hit
GATGCAGGTACAGAACACACTAGCTCATTCTGTAGTAGACAACAGGATAG10.9615384615384616No Hit
GGATATGAACAGATACTTCTCAAAAGAAGACATTTATGTAGCCAAAAAAC10.9615384615384616No Hit
GTCATAACGGAAATTATAAAATATTATTATATATTATAAATAATATATGT10.9615384615384616No Hit
CGTTTTGGCTGCTGAGTGCACAGGGTTGGGGAGGGGTGAGACCACTTGTA10.9615384615384616No Hit
GCCCAGGTGCTCTGCAAAAGACCAGGATTTAGTGGATGGAGTGGATGGTG10.9615384615384616No Hit
AGCAGGATCCCTGGCCTGTGGCTTCCGTTGGGTGTGGGCAATGCCACGTC10.9615384615384616No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG10.9615384615384616No Hit
CATTAGGATAAAAACCCAGATAGCCAGTATCCTCAGCTGCAGAATTACCT10.9615384615384616No Hit
GTCTCTTTCAATCCTCACAATTTCCGTTTTCACGGGTCAGTTCACTGAAA10.9615384615384616No Hit
CCCTTATAATGGACATCCACCATATATCCATGGAAAACCAAAAGGATACA10.9615384615384616No Hit
CCACAGGACTTAGGAGTTGGTTAACTTTGGGGGCATTCAGGGAGGGAGAA10.9615384615384616No Hit
GGTAGTAGCTTCCGGTCCTTTCGTAAACAAAGTTCTGAAAGTGCTTGTTG10.9615384615384616No Hit
CTCTAAGACCCAGAAGCCCGTCTGTGTCATCCTTTCCCCCCTGGAGACCA10.9615384615384616No Hit
CAAAACTCACAGAATGCAGTAAAACAGGTTCTAAAATGGGGCACCCGATT10.9615384615384616No Hit
CTTTTAGACCTGCACTGGAACTAGGGTGAGTCCCAATTGTCCTCATAGCC10.9615384615384616No Hit
GGGCAAACATAAGTCAATGGAGACATCATATTGGTCCCCAGTCCAGCAAG10.9615384615384616No Hit
CAAAGTGAATGACCAAGATATTCTAGGGAAAAAGTATAATATGCTGTGAA10.9615384615384616No Hit
CATCTGAACAGACTAATGACCAGTAGAGAGATTAAATAAGAAATCAAAAA10.9615384615384616No Hit
CAAAAACAGCCCATGGGGCTAATAATACAGTGTATGTTAATTTTTTAAAA10.9615384615384616No Hit
TGTTGAAGGAGTTCTTGGGGAGTCCCCGGTCTGACAGCGTGGTGGAGTTC10.9615384615384616No Hit
GCACAGGGGCGGCCCTGGGACATCCCCAGCTCGCCCCTGTGCCAACCTTA10.9615384615384616No Hit
TCGAGAGGGCGCATGATCAGAAGAGCTGTCCATGGTGACACCAGCCGTGT10.9615384615384616No Hit
CGCTAATATCTAATGATTTCACCTTCTACTGTGATCAAACATAATTGTAT10.9615384615384616No Hit
TTCTCACATAGAAACTTACACATTCCTCAGAACCATCTCGATGGCTTCTA10.9615384615384616No Hit
CTAAAACTAAAAAACATGCTACGGACCACCCAATGGGGTTCATTGGCAAA10.9615384615384616No Hit
CAACAGTAGCCTGAGCCTGACAGCCAGCACCACTCACTAACCCCCCCAAC10.9615384615384616No Hit
GGACCCAGCTGGTGGGTTCCACAGTTTCCCCCTGGAGTGTGTCACACACC10.9615384615384616No Hit
GGTCACTGAGGCCGGCATTCTTGGCAACATTAAATAAAGTCAACCCAACC10.9615384615384616No Hit
TCCAAGGCCAGGTAGTATGTAGGCCATTTCCCAGTAGTGTGACCCCACTT10.9615384615384616No Hit
GAACCGCCACGCGCACACACGCCTGTGAAAGCCTTTTTCTCCACCGTCAA10.9615384615384616No Hit
AACTGACTGAGCCACCCAGGCATCCCAGCAAATGCATTTAGATGATTACG10.9615384615384616No Hit
GAGAGGGAATGAACCAACACAAGACATGAATAGCATGACCATTTCAGACT10.9615384615384616No Hit
GGTGTAAGCTGTCCTCATGGGGAAAAGAGGAGAGGCACTGAGACCTGCCC10.9615384615384616No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers