FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_Neg-Control-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_Neg-Control-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA11.5625No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG11.5625No Hit
GTCATGTCTGGCAATATCCCAATCATTCCCATCACATTGTCGATAGCTTC11.5625No Hit
CTCCCTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATAT11.5625No Hit
TTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTC11.5625No Hit
CCATATTCCTTCATTGTCTTTTCCGCAAGCTCGACAATCATTGGATTGAA11.5625No Hit
CACCATGGACACAGTAAACAGACACATTCCTGGATTCCCAAGAGGAATCG11.5625No Hit
GACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGT11.5625No Hit
GGGAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACA11.5625No Hit
ATTCAAATGATCTTCTAGTTTGAATTTGTGTATCACCTCTGTGGCATCGG11.5625No Hit
GGCACTAATAATTAACTGATAATCACTGTTGTTATTACATGACCTCAGGC11.5625No Hit
CACCTGGGTGGCTCAGTGGGTTAAGCCTCTGCCTTCAGCTCAGGTCATGG11.5625No Hit
TCCTAAAGAAAAAGACATATGAACAGACTAATGACCACTAGAGAGATTAA11.5625No Hit
TTGTTGGCCTCGGGCCTCACGCTGGCGCCGCAGAGGGGGCACAGAAGCCC11.5625No Hit
GGTATCACCTCTGGCTGTGACATGGTGGAATAGGAATGGACAAATAACAA11.5625No Hit
AAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGATGG11.5625No Hit
TCCCAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC11.5625No Hit
CATCTTTATTGGAGATGTGCCACAGCACACAGATTGGTGGAATTAGGATG11.5625No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG11.5625No Hit
CTAAAGAAGTAAAATAAGCAAGACATCTGATAATGGTGAAGCGATAGTTT11.5625No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.5625No Hit
CCAATAAAGATGCTAGTGGATCTGCTGATACTGCAGCTCTTCTCACTATG11.5625No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA11.5625No Hit
GCAAAGTAGAATGCAGTTCTCCTCATTTACTGTGAATGTGAGGGGATCAG11.5625No Hit
TGTAAGCAACCATCAAAGGAGAAATTTTGCAAACCTGGAGTTCTTGATTA11.5625No Hit
GAATTCCCCTTTGGCCTGTGTTGAGAATAGAACGATTCCTCTTGGGAATC11.5625No Hit
GATACTAACATCGGAATCGCAACTAACGATAACCAAAGAGAAGAAAGAAG11.5625No Hit
TCCGAGAACTGTGAGTCTCTTCGTGGCCTTGTTATAGTTGAATACAGGAG11.5625No Hit
GTGTAGGGGGAGGCCCTTTGATTCTGCCGGGCTGATGAATGTGTTCTTGG11.5625No Hit
GGATACACCATGGATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGA11.5625No Hit
CGCTGTTAAGATATCAATTGAATGATCCCAAGAAACCCAAACTGCCTACA11.5625No Hit
GGTATAGGGAGCACAGTGGTATAGGGAGCACAGGCTGCTCCCTGTGTCAG11.5625No Hit
ATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCAT11.5625No Hit
AGGCATAATAAAGGTGACTAGGTCAAACTACTAAAGATAATATTTATTTA11.5625No Hit
ATATGGTAATTGACGGCTAGATTCCTACATAGAAGCTAACGGGGCTGACG11.5625No Hit
GTCTCCCACCTAACTTCTCCAGCCTTGAAAACTTTAGAGCCTATGTGGAT11.5625No Hit
ATGTACCACTGTTGGGAGATCGACAGGCAGTTGTTGACCACCCAGTACAG11.5625No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT11.5625No Hit
GCTCACAATGCCATCTCTCGGGGTAGGGATGGCCTGCCCTTTTGCTCTGG11.5625No Hit
ATATTGATTTTTCCTACCCGTGAGCGTGGAATGTTCTTCCATTTGTTTGT11.5625No Hit
TAAGAGACAATGAGGGATATGAAGACAGAACAATGGATGGGAGAAGAGCA11.5625No Hit
CTCCTTTGCAATTTATGTTAAGTATAAGTTATAAAATATAATTCTTTTAA11.5625No Hit
AATCAATAATTCATTCAAACGTATTTTCACAACATCAAAGTATCATAATA11.5625No Hit
ACCCATAGGTGAACTTCCTTTCACAAGAGCAGAATCATATGGGCTAGCAA11.5625No Hit
TGATAAAATAACATACAAGGTGAATAATATATTCTACAAGATGAAAAAGC11.5625No Hit
GTGTTTCACTGACCTCCTCGGGAGACAGTAGTACATTTCCTCGTTGGTCC11.5625No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCAATTTTGATGATT11.5625No Hit
GTTGACATAAATCCTGGTCATGCAGATCTCAGTGCCAAGGAGGCACAGGA11.5625No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA11.5625No Hit
CCAATAACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGA11.5625No Hit
GCTAACACACTCCTCTACATCTGGTGTCTTGGCTTCCTGAGGTGACTCCT11.5625No Hit
TACAAACCCACTGTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAA11.5625No Hit
CATTCACAGTCAATGAGGAAAAATGCATCCTACTCTGTTCCATAATCACA11.5625No Hit
CCGTTATTAGCCAGAAGTCAGATGCTCAAGGGGCTTCATGAGGTCCGCAG11.5625No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG11.5625No Hit
GGTCTGACCCTGGCCCGTGCCAAGCTGACCAAGAACTATGTGCCTGCCGC11.5625No Hit
AGATAATAGCTCAAATAAAAAACTAGAGTATAAATCTGTTACAAGAATGT11.5625No Hit
GTGTCAGGCAGGAAGTAAGTATGCTAGAGTCCCCTATGCGGGTAATAGCC11.5625No Hit
GTTTAAGCTGTTGCTAAACAAGGAACAGGGATACCATAATAAATAGGAAA11.5625No Hit
CTTTAGCCTTTCGACTCTTTCAAAATAAGTTTTGTAGATTTTTGGATAAT11.5625No Hit
GTGGCACATCTCCAATAAAGATGCTAGTGGATCTGCTGATACTGCAGCTC11.5625No Hit
GAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAA11.5625No Hit
GAAAGGATGTGAACGAACTGGGCGAAAATGGACAGAAGGCAAGAAAGAGT11.5625No Hit
AAAGAAGGTAGTAGTCTCTCACTTGCTGAAATCTGAAAAACAAGGGACTC11.5625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGAAG50.0145.0145
TGATTTT50.0145.05
TTTTCCT50.0145.09
TTTTTCC50.0145.08
TATTGAT50.0145.02
TTGATTT50.0145.04
ATTGATT50.0145.03
GATTTTT50.0145.06
ATTTTTC50.0145.07
ATATTGA50.0145.01
Produced by FastQC (version 0.11.5)