FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_HK_1073_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_HK_1073_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGGAAGAGGTGCTTACGGGCAATCTTCAAACATTGAAGATAAGAGTG13.225806451612903No Hit
CTGTGAAGATTGGGTGGTCGGGGGGGGGTTCGGGGGGTGTCGGTTAGGGG13.225806451612903No Hit
GAGGGGATCAGGAATGAGAATACTTGTAAGGGGCAATTCTCCTGTATTCA13.225806451612903No Hit
GTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGATGTATACTCCAGG13.225806451612903No Hit
GTGAGGAATGATGATGTTGATCAAAGCTTGATTATTGCTGCTAGGAACAT13.225806451612903No Hit
GGGCACAGATGTATACTCCAGGAGGGGAAGTGAGGATTGATGATGTTGAT13.225806451612903No Hit
GACTGAAATGGTTCATATTCCATGTTATTGGTTAGGATTGTAGGGTGCTG13.225806451612903No Hit
GCCTAAGGATGTCTGCCATCCTAATTCCCCCAATCTGTGTGCTGTGGCAC13.225806451612903No Hit
TTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGATGTATACT13.225806451612903No Hit
ACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGATGTAT13.225806451612903No Hit
CAGATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAG13.225806451612903No Hit
GTGCGGCGCGGGTGGGGGGGGGGGCGGATGGGGGGGGGGGGTGGGTGGGG13.225806451612903No Hit
GTTCCATGCTTTAGCCTTTCGACTCTGTCAATATAAGTTTTGTAGATTTT13.225806451612903No Hit
AGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGCGATTGAATC13.225806451612903No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTGCGGTTGATTACCAACCCCCCGG13.225806451612903No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC13.225806451612903No Hit
TATGAAAATGTCTTAAAAGTTGATGCATAGGATTCAATCCCTGATTCGCC13.225806451612903No Hit
CAATCCTCTTGTGAAAATACCATGGCCACAATTATTGCTTCGGCAATCGA13.225806451612903No Hit
CTTAAGTGTGAATCCACCGAAGCTGGGGGAGGAGGTAAGTGTCGGGCCCA13.225806451612903No Hit
GGATCCGCCCGAGTGATGGTCTCCCCGCGGGCGGTGCCCGGGTGGCCGAG13.225806451612903No Hit
CTCCCAGCCATTGTGAACTCTTCATATCCCTCATGCACTCTTATCTTCAA13.225806451612903No Hit
GAGGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAG13.225806451612903No Hit
GGCCATTAGAGGCCAATACAGGGGGGTTGTAAGGGCTCTGGGCCAGCAAG13.225806451612903No Hit
AGCATGTTCCTTGAGTCAAATGCAACACTTCAATGTACACACTGCTTGTT13.225806451612903No Hit
GGGCGAATCAGCGATTGAATCCTATGCATCAACTTTTAAGACATTTTCAG13.225806451612903No Hit
GAATAAATACACATAGTCTCTAAAGTTTTAAAGGCTGGAGAATTTAGGTG13.225806451612903No Hit
TCCCAGAACCCTACAATGCTATACAATAAAATGGAATTTGAACCATTTCA13.225806451612903No Hit
CAAGAGGATAACGGAAATGATTCCTGAGAGAAATGAGCAAGGACAAACTT13.225806451612903No Hit
TCTGTTCCCAGCATGTTCCGTGAGTCAAATGCAACACTTCAATGTACACC13.225806451612903No Hit
CATCGCGATGCTTGGAGTCATGTCTGGCAAGATCCCCATCAGTACCATCA13.225806451612903No Hit
CCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCATCTGGGTCTTCAG13.225806451612903No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers