FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_HK-1073-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_HK-1073-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748179
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT40560.5421162582750919No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT39930.5336958134350203No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA39230.5243397636127184No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC34380.4595157041296267No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG31070.4152749542555993No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG29800.3983004067208516No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT29420.39322140824588775No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT24290.3246549288338753No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC23820.3183730096674726No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC22800.3047399084978327No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA22140.2959184900939481No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA21310.2848248881617902No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG21030.2810824682328694No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT20820.27827565328617887No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG18390.24579679461733087No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC17350.2318963777384824No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA17340.23176271988387806No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA17310.2313617463200651No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA15830.21158038383862687No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC15220.20342725470776377No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT14540.19433852059467052No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC13550.18110639298884357No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC13150.17576007880467107No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC13120.17535910524085815No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG12940.17295326385798052No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG12780.1708147381843115No Hit
ATTCTGCATTATATGCCCATACGTCTTGTATTTGGTCATCAATCTTATTA12710.1698791332020813No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG12490.16693866040078645No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG12440.16627037112776488No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG12370.1653347661455347No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG12280.16413184545409587No Hit
GTACATTGCCATTCCACAATATCAGTAAATATGCATTTGGAACCTGCCCC12230.1634635561810743No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG12210.16319624047186568No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG12210.16319624047186568No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT12060.161191372652801No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA11950.15972113625215356No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA11750.1570479791600673No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA11370.15196898068510345No Hit
AAACAGTACATTGCCATTCCACAATATCAGTAAATATGCATTTGGAACCT11370.15196898068510345No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT11340.15156800712129048No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA11110.1484938764653913No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA11050.14769192933776543No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG11040.1475582714831611No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG11040.1475582714831611No Hit
ATCATATTGAGTCTAGTTTCAACCTCACTGAATTCATGATCAATTATTTC11010.14715729791934817No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC11010.14715729791934817No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG10960.1464890086463266No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC10890.14555340366409641No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG10760.14381585155424037No Hit
TTATATGCCCATACGTCTTGTATTTGGTCATCAATCTTATTATTGATCAT10730.14341487799042743No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA10230.1367319852602118No Hit
GTGTAGTGCAATGTCAGACTGAAAAAGGTGGCTTAAACAGTACATTGCCA10170.13593003813258592No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC10140.13552906456877298No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA10120.13526174885956435No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC10080.1347271174411471No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT9990.13352419674970828No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA9740.13018275038460048No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT9670.12924714540237028No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT9650.12897982969316166No Hit
CTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGTTC9600.1283115404201401No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG9500.126974961874097No Hit
ATCTTATTATTGATCATATTGAGTCTAGTTTCAACCTCACTGAATTCATG9390.12550472547344954No Hit
GTACTAGCAATTCTGCATTATATGCCCATACGTCTTGTATTTGGTCATCA9090.12149498983532016No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT9030.12069304270769428No Hit
GCACTACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTCCATGG9000.12029206914388134No Hit
ATGTAAGAGTTAATAGTCTCAAACTGGCAGTCGGTCTGAGGAACGTGCCT8960.1197574377254641No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC8770.11721793848798216No Hit
GTCTTGAACAGGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCC8730.11668330706956491No Hit
CATTTATGGTATAGCTCGAAACAGCCTTTCCCATCTTCCATAGCATTGGA8710.11641599136035627No Hit
GTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACCACCTT8660.11574770208733472No Hit
CTAATAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTG8440.11280722928603984No Hit
ATTATATACAAATGTTGCATCTGCAAGATCCATTGGACATGGCCCAGAAC8390.11213894001301826No Hit
ATTTGGAGCCATAGCTGGATTCATAGAAGGAGGTTGGCCAGGACTAGTCG8320.1112033350307881No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA8270.11053504575776651No Hit
ATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAGACACA8260.1104013879031622No Hit
CTATTAACTCTTACATATTTGGGGCAGGTTCCAAATGCATATTTACTGAT8260.1104013879031622No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC8230.11000041433934928No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT8170.10919846721172338No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG8010.1070599415380544No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA7920.10585702084661558No Hit
ACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTCCATGGCTCCC7860.10505507371898971No Hit
GGCTTAAACAGTACATTGCCATTCCACAATATCAGTAAATATGCATTTGG7800.10425312659136383No Hit
ATCCAATGGGAATCTAATTGCTCCATGGTATGGACACGTTCTTTCAGGAG7680.10264923233611208No Hit
GATAAAATCTGCATCGGCCACCAGTCAACAAACTCCACAGAAACTGTGGA7620.1018472852084862No Hit
CCATTGGACATGGCCCAGAACAGGAAGGCAGCAAACCCCATTGCAAGCAC7560.10104533808086033No Hit
ATTTCATATTGCTTGTTCATCTTGTCGACTATATTATTCACCTTGGATGT7520.10051070666244309No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTCG100.0070906254144.99919145
AGTAGAA7100.0117.4760361
GCAGGTA3000.099.096022
GGTACTG3150.092.069145
TTACTGG259.383533E-487.005334
CCACACC608.2945917E-1084.58287145
AAATATA2800.082.862225
CTATAAC353.435665E-582.862225
TCTATAA353.435665E-582.862224
TTTAACA353.436804E-582.856689
GGTCAAA2750.081.7596051
CAGGTAC3650.079.462233
AAAAGCC651.5625119E-978.086932
AGAAACA10950.076.146224
CGAAAGC1750.074.5809863
TAAAAGC702.8030627E-972.528691
TATAACT406.662274E-572.4995966
GCGAAAG1900.068.6930162
GTACTGA4300.067.441486
GCTTTAA6250.067.306621
Produced by FastQC (version 0.11.5)