FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1987_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1987_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTACACGTGCGCGCGCACACACACTCACACGGCCATGTGAACCCAGG13.4482758620689653No Hit
ACTTAGACCATCAGAGAAGGTGAACCCTGAGCTAGCTATCCATTCCAGGA13.4482758620689653No Hit
GGCGGATATGTCCCCAGCAGCGCCCGCCGCGGCGGCCCGGTGGGGGCGGG13.4482758620689653No Hit
GTCCCATCTGTCCTCTGCTAGAGGAGGTGAACTCCTGGGCTGGGTGGCCC13.4482758620689653No Hit
CCATCAAACCTGACCTGGGTATAACTGTATACTGTGATAGTACCCAATAT13.4482758620689653No Hit
CTCGGATGCTTTGCCACATAGAGTTTAAGGAAGAATACTCTTAAAATGCG13.4482758620689653No Hit
CTCATGTATAGGACCCTCAGGGGCCCACCAGGATAATCCAGGACAGGCTC13.4482758620689653No Hit
CAGGTGTTTAGTCTCTACCAGCAGGAGGACACAGATGGAGTCTACCTTTC13.4482758620689653No Hit
GAAGGGGTGTTCTGGGGTTCTCTCTGATGGGCTGGTGGAAGATGGAGGAT13.4482758620689653No Hit
CTCCTGAAGGAACGTTGAAGACGACGACGTGGATAGGCCGGGTGTGTAAG13.4482758620689653No Hit
ACCTGCTCCTGTCCCAGTCTCCCCATCTTAGTAAACAGCATCCCCACCTC13.4482758620689653No Hit
GGCAACCTGCCTGGCGTAACAGTGCTGTCAGAACTGTCAGAGTCGATGCA13.4482758620689653No Hit
AAGTTGCGCGGGGTTGTGGAGGTTGTGTTTGGTTTGTAGTCGCGCGGCAG13.4482758620689653No Hit
CTATAATTACAGTAGCTTTGCCTACCCTAACATGAAAGGTTATGCTAGGG13.4482758620689653No Hit
GCAAAGCCTAAAACATCTACTATCTCCATCTGAACAGAAAAACTTTGCCA13.4482758620689653No Hit
GCGGTAATTCTACGGAATTAAAGGTTGTTTACAAACTTGCCGTCGACTGT13.4482758620689653No Hit
CAACCTCGGCGCGTGCCTGCGTAGCGCGGACGCCGCTGGTGTTCATGCGG13.4482758620689653No Hit
GCTATGCAATTTGTACGTTAGGGTGTAGTTTATTTGTCCTTGAGTGAGCA13.4482758620689653No Hit
CTCTGTTTCTCTAGGCTTGGCGTCCTCTGACCCACGAGCAGCCCTGGAAG13.4482758620689653No Hit
ATGTATTACACTTCACGGAACTACGTTCCTTTAAAATGCACTTAGAAAGC13.4482758620689653No Hit
CAGTAGCTCTTCCCAAATTCTGGGTCAGTTACCTAACTCCTCTGCGCCTC13.4482758620689653No Hit
GTGGGAGGCCGGACACCTAGCAGGGGCTTGCCAAGGGCTCAGAGCACAGT13.4482758620689653No Hit
AAACTTTCGAATCTGGCATCTCTGGGACAGGAAGCAGAGATCTAACATCC13.4482758620689653No Hit
AAAGCAGGGCGTAGGAGCGTGCTGGCTTGTCCCGTCCTGGCGTGCTGCCC13.4482758620689653No Hit
GTGCTCGGGAAAGGAAGCTTTGGCAAGGTATGCTGTGGCGTGGGGGTGGC13.4482758620689653No Hit
GACTTAGAGTCTCCTGAAGGAACGTTGAAGACGCCGACGTTGATTGGCCG13.4482758620689653No Hit
GCCCAATATGGGACTCAATCCCAGGTCTTCGGGATCAGGACCCGAGCTGA13.4482758620689653No Hit
GAAGCGTACAGCGCGCTGAACGCAACACCGTTTACCGACAGCCTGGCGAT13.4482758620689653No Hit
CCTCTGTTCCATGCTCGTGCAACTCTCACATCGGAAGTCGTTAGTCTCAA13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)