FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1987-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1987-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905696
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC2850.050.882543
GCGAAAG3800.038.1661222
GTGATCG1553.4699042E-837.4191559
AGCGAAA3700.037.2439231
ATAACGC1001.3913894E-436.249818
TGATCCG900.003628146432.222059
GACGATG3450.031.52157145
GTAATAT3750.030.9451271
GAGCGAA950.00471825630.5379541
GTATATC1000.00606285629.0110571
CGTAGAG1255.146963E-429.0062522
ATACAGC5900.027.0337546
TATACTG3607.2759576E-1226.1804165
GCGTAGA1409.971653E-425.9027291
GTATAAT4550.025.5042271
TATAATA10100.025.1291772
CTAGCAC6000.024.1692053
AATAACG1550.001808414523.3869727
TGTACCG4006.89397E-1021.7498865
GTATATA6100.021.40161
Produced by FastQC (version 0.11.5)