FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1987-d10-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1987-d10-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219747
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC400.00604744854.3835333
ATAACGA1100.00967273726.36345145
AGCGAAA3900.026.0393351
GCGAAAG3950.025.7023432
GTATAAG4600.023.6537421
TATACGG1550.0018087923.3869325
GTATTAT14300.021.304911
GCACCGT10550.021.3031676
CGAAAGC5251.8189894E-1220.7175373
TATACTA3157.2849616E-720.7141385
ACCGTGT10500.020.0236668
TATATAC8950.019.4443913
GTATAAT6350.019.419661
AGCACCG11250.018.0443175
TATACAT10200.017.7694825
ATACTAT3703.4002423E-617.635016
GTTATGT8250.017.5850051
GTCTTAA6653.6379788E-1217.4527871
GTCCTAT12100.017.3851761
TAGTACC6303.6379788E-1117.2617829
Produced by FastQC (version 0.11.5)