FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1987-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1987-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965469
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG22460.1142729801385827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG2103.783498E-1034.5235339
ATATCGC1000.006070066229.0056693
AGCGAAA6350.028.5561521
GTTGACA20500.027.95161
CGTAATA1050.00770096827.6258542
CTACGGC1050.007702880527.6244473
GTTACGT3005.838956E-1026.5952971
GCGAAAG7000.025.8992372
CGAAAGC7050.025.7142453
ACATGGT21600.025.1753315
GTATATA8650.024.3172971
TATACTG8500.023.8839845
TGACATG25500.021.8964373
TAGGTTA5900.019.662365
GTTATGT13300.019.6328931
CTACTGA21350.019.3563086
GACATGG28200.019.2847024
TAGCATG24900.019.2216032
TACTGAA22750.019.1207247
GGTCCTA33000.018.8937879
Produced by FastQC (version 0.11.5)