FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1987-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1987-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246953
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG951.02905215E-438.15785
TACGGGG1301.6551361E-533.4614527
GCGAAAG4100.031.8394031
ATATACG1000.00607509828.9999284
GTGATCG3900.027.8845449
TAGTACT3153.45608E-1127.618984
CGAAAGC4950.026.3646282
TACGGTG1500.001494501324.1666075
AACCGCC1952.61529E-422.3076367
TATATAG4151.0932126E-920.9646452
GAGACGC1750.003659282620.7142359
GGGCGTA1750.003659282620.7142357
TAGAGTG5750.020.1738625
TAACCGT2908.252351E-619.999955
GTCCTAT8800.019.7790221
AGCGAAA4402.2555469E-919.7790221
AATCCGT2659.3522176E-519.1508966
CCCTACG3051.2749431E-519.0171092
CCGTAGA2701.0768084E-418.802281
GTATAAA9300.018.7156331
Produced by FastQC (version 0.11.5)