FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1987-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1987-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022226
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC11600.1134778414949336No Hit
GTGTTACATGTCCCCAACCCCTCACCCTGTGAGGCGTGTGTCCCTGAGAC10270.1004670200131869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4200.050.064252
ACACGAC1501.0913936E-1148.33317145
GTATCCG2650.041.0375989
CTTCGAC1303.6971505E-739.0402373
CACGCCG800.002036669836.2498785
GTATAGG4050.032.231571
AGCGAAA6400.031.7279551
CGCCGAG950.004727421330.5262137
CGAAAGC6900.030.4724033
ACCCTCG3201.8189894E-1229.4530267
CCGATCG200.00607686228.99990310-14
CGATCGA200.00607686228.99990310-14
TTCGACA1804.6984314E-628.1957284
CAGCGCG1804.7002195E-628.194359
ATAATAC13950.028.0657943
TAATACA11800.027.035134
GTGATCG2156.124992E-726.9766549
TACAGCC9000.026.5832427
ATAAGAC4450.026.0686043
TAGCACC4800.025.6782534
Produced by FastQC (version 0.11.5)