FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1986-d10-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1986-d10-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292448
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCATTACCTTC37350.2889864814677264No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC30020.23227240090123547No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA28920.2237614201886652No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT28200.21819059644952832No Hit
GATACTACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCT24130.1866999678130184No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG22360.17300502612097354No Hit
GTTCTGGACCCTCCCTTCTCTCCTCCAGGCCCAGGTTCCGGCTCCCGCTT21020.16263710416202432No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT20620.15954220208472603No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC20610.1594648295327936No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA19540.1511859664760207No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG18880.14607937804847854No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG18120.14019906410161184No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG17130.13253918146029858No Hit
GAGAGCATGATCGAGGCTGAGTCTTCTATCAAAGAGAAAGACATGACCAA15680.12132016143009236No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT15450.11954059273564584No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT14710.11381502389264403No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG14570.11273180816558964No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA13820.10692886677065537No Hit
CCTCAAGACCATGAGAAGTCCCTGCTGGCTCCCTTCCTGAAGGTTCTTCT13310.10298286662210007No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA13260.1025960038624378No Hit
ATGTAGGACTGTCCCCTCCCCAAGCAGAATGAGAGACCTGTGCTGACAGA13210.10220914110277551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG3150.096.6697855
CGAAAGC6050.074.305673
GCGAAAG6550.069.7431642
GGACGCC350.003566830262.14246145
GTACTGA5850.059.4867976
TTTCGTT502.010529E-457.999626145
ACTCTAA1900.057.236473145
GACAGTA4800.055.885067
AGCGAAA8850.051.637811
AGGTACT6250.051.043624
GCAGGTA6150.050.698582
GCGGTAT5800.049.9996836
TATAGCG5850.049.582
CAGGTAC6900.045.186113
CGGTATC6450.044.9609537
AGGACAG5000.044.951455
ATTATAC2750.044.8230973
TTATACT13400.043.827744
CTGATCG3350.043.2833069
GGATAGC8400.042.2930345
Produced by FastQC (version 0.11.5)