FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1986-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1986-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473383
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC63900.43369578717821505No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG45770.3106456366063678No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT36960.25085127220824455No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG34830.23639474596897073No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA30920.2098571790226981No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA30600.2076853065360466No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA27490.1865774208064027No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC25570.17354618588649387No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT24340.16519805101592727No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC23710.1609221770578322No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT20620.13995003335860398No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG20190.13703157970466606No Hit
CCTTCCCGATTGAGCCTTCCTCCACTCCCTTAGGTGACTCTCCAATTGGC19620.13316293183781813No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG19160.13004086513825663No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA19090.12956576803180164No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC18830.12780112163639734No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC18430.125086281028083No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA18320.12433969986079656No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC18120.12298227955663937No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT17770.12060679402436433No Hit
CCCTTAGGTGACTCTCCAATTGGCCATGTCTCCGATCTGTTTTCAAAGAA17710.12019956793311717No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA17510.11884214762896003No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG17510.11884214762896003No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC17320.11755259834001072No Hit
CCTTTACAGTTACCGGAGACAACACCAAATGGAATGAGAATCAGAATCCT17150.11639879108147713No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC17090.11599156499022996No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC16650.1130052403210842No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT15760.1069647199675848No Hit
CCATAAGACAGGAAATGGCAAGCAGGGGTCTATGGGATTCCTTTCGTCAG15300.1038426532680233No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG15290.10377478225281546No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA15250.10350329819198403No Hit
GTACATAATGAAGGGGGTTTACATAAATACAGCTTTGCTCAATGCATCTT15070.10228161991824257No Hit
CAGATACACCTACCGATGCCACAGAGGTGATACACAAATTCAAACTAGAA15030.10201013585741114No Hit
ATTATGTACTCAGTGGCCCTGCAATGAGACACTTCCGCTGTGAAGTAGTT14840.10072058656846183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAAGTT650.0111.53818145
AGTAGAA3550.098.0611951
GGTACTG2050.084.877835
GACTTAA554.3959517E-879.090706145
GCAGGTA2300.078.8068162
AAGGTAG1050.075.952195
TATTCCT1450.069.999825
GGTAGTT1200.066.458167
AACACTG6650.065.413365
CGAAAGC808.090865E-963.4373363
AGGTAGT1300.061.3459976
CTTCCCG7100.061.2695272
CCCGATT7150.060.839015
AATAACT2900.059.9998443
ATACCCC851.3060344E-859.70573145
TAACTAT5000.059.4700281
CAAGGTA2100.058.6903239
AGAAACA5950.058.4872444
TTCTACT753.7625068E-757.9998474
TCCCGAT7600.057.2366944
Produced by FastQC (version 0.11.5)