FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1986-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1986-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057213
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAG1701.8299033E-938.382114145
GCGAAAG5900.029.498322
CGAAAGC6000.029.0025623
TTACGCT1000.00607339329.001194
GTCCTAT7900.026.6276131
GTTACGC1100.00966766326.3659693
GCAATAC1902.191972E-422.896763
CGTCGTG1600.002175310422.6561099
TATACTG4256.184564E-1122.1763345
CTATTGG2653.7634309E-621.898051
ATTATAC3055.3362237E-721.3953343
CTATAGA3851.0451913E-820.7249371
GTTGACG1750.00365899920.714157145
GTTATAG5650.020.5415331
TCCTATG10800.020.1435282
ATACTAT3001.100726E-519.3432751
TATACCC5254.9112714E-1119.3341274
CTATGAT9450.019.1806834
GTATATA5407.4578566E-1118.8059641
CTATATC7400.018.615634
Produced by FastQC (version 0.11.5)