FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1986-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1986-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAAATAATTTCTATCATCAGGCAGATTTTATGGGGGGGGGGGGGGGGG11.25No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC11.25No Hit
CCTATAATGTTCTCAAATAAAATGGCGAGGTTAGGAAAAGGATACATGTT11.25No Hit
CTCTAAGATGTGATGTGAAAATAATTTATATAGAATGAATAAACATGACT11.25No Hit
CTGTTGCTGTCCAGGTAGAGCTGGTGAATGGCCGTGGGCACCTGGGGGGG11.25No Hit
ATATACAACGAAGACTTTTCACTAAGTACCGGATAGAATGATTCCTCGCT11.25No Hit
AAACAAATAACTAAATAAAAAATAAGACACAAATTCATTAACTAAATAAT11.25No Hit
TACAAAAACCAAAAGGATATTATATCTAACGATTAATAAAAAAAAATATA11.25No Hit
TCACTGACTTCTTCGGGAGACAATAGTACGTTCCCTCTTTGATCTCTAAC11.25No Hit
TTCTTTTTTTTTTTTAAAAAAATCACTGAGAACCCAAAGCAGTGAATTGC11.25No Hit
CTTTAGATGTATTCAGAGCGGCTTGTGGGAGGGTGGAACGGGAAAGCATG11.25No Hit
GTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGT11.25No Hit
GTAGATACATTTGTTGAATGAATATTTGAGATGAGGAAACTGAAGTTCAA11.25No Hit
TAGTGCTCCAACCCTACCTCAACAAACAATACCCATCTCAAATAATCAAC11.25No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG11.25No Hit
GTTTAACTTTGTAACATTTAGTATTAAAGTTTACTTATTAGTCTCTCTCT11.25No Hit
CCTTACATATGATCTAAGGTGGAGAACGTTCTAGGGGCACTTGAGAGAAG11.25No Hit
GATGTACTGGGCGTCCGCCATGGGCCCTGCTGAGAACCAGCGCCATCATC11.25No Hit
ATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATTTG11.25No Hit
GGCTAACCCATGAAAAGGGAGATTTTCTGCCTTCAGCAGTATTTCCTTAG11.25No Hit
GGACTAGTTAGCTCAATTGTCCATACCTGCTGAGATGCCCAGTCCATCTT11.25No Hit
GTACACATCCAGGGGCCACTTGGGTGAAGGCAGAGCACGGGCACTCCAGC11.25No Hit
AATTAGAACAGGGGTGAGCTTTGTTCATGGCTGCAAAGCTAACAGATGAT11.25No Hit
AAATGAAACAAGCTATACTTATCTTATAATTTAGTGCACAATAAGCAAAA11.25No Hit
GATCCCAGCCGAAGACACCGGCTTAACCAACTGAGCCACCCAGGCGTCCC11.25No Hit
CAGATCATGAGTCTAATGAGTTATATTACAGGTTCTGCCCCAGGCCCTGT11.25No Hit
GGCCTGCTGCGCCCCACCCACCGGGTGCGCAGGGGCTGGAAGCCCGGGAC11.25No Hit
CCCATAAAGCCATCGAACACTAACAGGATACACCATGCACACAGTCAACA11.25No Hit
CGATCTGACCGGGCAGGGATGAGAATTCGAAACTTGCGGAAACACTGGCC11.25No Hit
GGCAGTGCACAGTTAATGAATGTGCATGTTGAAGGGGGGGGGGGGGGGGG11.25No Hit
GACCAAAAGAATTCGGATGGCCATCAATTAGTGTCGAATTGTTTAAAAAC11.25No Hit
CTATGACAAGTGATGCCCCCTTGTCCCGTGTCCCAGTCTTGGCCTGATCT11.25No Hit
TTTCTATGTTCATTGGCTTTTGGTCCATCTTCCCTACTAAGCTGTAAGCT11.25No Hit
AAACTAAACTATTCAAAGTTAAACACGGAAGAAAATATAATAATTGAAGG11.25No Hit
GGTCGCTGTATGGCGTCCCCCCTCCGTGGCCAGATGGACACAACGGCACC11.25No Hit
TACTAAGATTGAATCCTAAAGAAAAAGAAATATGAACATACTAATGACCA11.25No Hit
GACACTTAACGACTGAGCCACCCAGGCGCCCCCAGATGAAATACATTTCT11.25No Hit
CTTCCAAATCTCTCTCCCCCTCTATCTTTGTGTCAGATTCTAACTTTGAC11.25No Hit
TAAGATGGCTGTACTTATGCTAAAGCTAAACCTTGATTTTCTTGGCTTCC11.25No Hit
TTACTGGATCATACCTCTTCCCGTATTGTACAATTTGGTTTGCAGATATT11.25No Hit
CAATGAGCCTGTGACAAGAGCAAAGGGAGGCAACCTTGAAGTTTTTCCAT11.25No Hit
TCTAACATAAACCATATAAACAATTCCATAAGAATCGCATAACACAATTT11.25No Hit
GCTTTTGGTCCATCTTCCCTACTAAGCTGTAAGCTTCATGAAAACAGGAA11.25No Hit
GAAACGCTCCCCACCTCGTTTTATGAGGCCCGCGCGGCCCTTATCGAGCC11.25No Hit
AAAAATACAACAGAAAGCTACTAATTCTGACCACCCCTATCAATTGCAGG11.25No Hit
GCCCTGGGGAGACTAGTGAAGGAGGATTAGGCTGAGATAACGTGTGAGAC11.25No Hit
CTAAAAATAAAAAAGAAAAAAAAAAAAAACCACCCATTCATCCTTTCATT11.25No Hit
ACACAGGAGGGATGTCACGTGGCCTCGGTGTCCAGTGGCCTCAGAGCCCG11.25No Hit
GCTGTAATCAAATAACACCTATCACAATTCATTGCATTTACGTCCATCTT11.25No Hit
ATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGTGA11.25No Hit
CCTCCGAGCTCTGCAAACACTGGCAGTGATTCAAGTAGCCACAAACACCA11.25No Hit
CATATTCATAGCAGCTTTATTTGCAAACGCCAAAAGATGGAAGCAACCCA11.25No Hit
CAGTCTCTCTCACTCCCAACCTCTGTGTCAAATAAAAAAATGGCATATTT11.25No Hit
AATTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.25No Hit
GTGAGGATGCCACCATGGACGCCATCGGTCTGAATCACCAAGCAAAGCAT11.25No Hit
GCGATCAGGGCCCTGCGCATGGCAGGTGTTCAGTAAGTGGCACCCATGCT11.25No Hit
GGCTCCCTGCTGAGCAGAGAGCCCGATGCGGGGCTCAATCCCAGGACCCT11.25No Hit
GATCAAAAGTAGGCAGAGAGGCAGGCAGGGGGAACAGGCTCCCTGCTGAG11.25No Hit
GCACATTACATGCGCCTGCCAGCAAATCCATCGTGGGTTTTTTTTTTTTT11.25No Hit
GTTGAGGAGCGAAATCGAACAGTTCGGGGCCGTGACCAGGGGCGAGCTTG11.25No Hit
GTGTGTGCTTAAAGTGCCTTAGGACACAAATTGCACACCACTGAGCAAAA11.25No Hit
TATTAACGTAGAAAAAGAAGACATAATTTTAGAGTGATCGCTTACACTGA11.25No Hit
ATTTAATCTATCTACTGGTCATTAGTCTGTTCAGATGTCTGTTTCTTTAG11.25No Hit
ATATCAGAGACAATAAACCAATGGTAAAACTGCTTCAAAAGTAAAGATTG11.25No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT11.25No Hit
CTTCTAGGACCATGAGAAACAAATGTATGTTTTTTAAGCCACCCATTCTA11.25No Hit
GAGCAGGCAATAAGGCTTACTGCACACAAATATCTACTCTTGGAGAACTC11.25No Hit
ATCTTCTCTTTCTTTCTTTCTTTCTTTTTTTTATACAAAAAATCAATGAT11.25No Hit
CCATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATTCAGTAACGTTGGG11.25No Hit
TGAGAGAAGTGTGAGGTATTAAAGTTCCCTATGATATTGGTGCTGATTGC11.25No Hit
ATTTTAAATGCCAACCTTTTAAAAACAATCACTGTACACAGCTCATCTCG11.25No Hit
GCCATATACTGTCAAGAGAAATGTGAACAACTGTAAAGACACAGTATTAA11.25No Hit
GTATCAGACTTGCACGACAGATGCCATGACTATCCTAGCTGGGGTTGGTG11.25No Hit
CAGCAGAAGTGTCCCACTGCAGGGCTACTGAATACATAATGAAGGGAGTG11.25No Hit
GTCCAAAAGTTCTACCCGCGGCCTGGAAAGGGCCTTCCCGCCTCCCTGCC11.25No Hit
AAGACGACTCCCCACTGAGCAGGGAGCCTGACCTGGGGTTCGATCCCAGG11.25No Hit
GTTCTGATCTCATACTTCTACTAACTTTGGAATTTATTAGTTCATATCTA11.25No Hit
TGTCACTGCTCTACTTGGCTCTCCCAGGTGGGGGCATGATATGGAGGCGG11.25No Hit
TTACAACCCAGTGCAGGTGTTTTTGAATTATGTCTCTCTTCCATATGGTA11.25No Hit
TCCCAGTGCTCCCCACTGGCCAAGTACAACTGAAGACCATTGGTATAGTC11.25No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGAAA50.0145.09
CCATGAA50.0145.08
AACCCAT50.0145.05
CCCATGA50.0145.07
TAACCCA50.0145.04
GCTAACC50.0145.02
CTAACCC50.0145.03
ACCCATG50.0145.06
CAGACCC50.0145.0145
GGCTAAC50.0145.01