FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1985_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1985_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGAATGTACTATGAACAATTACACACCAACAAGCTGGATAATACAAAA15.0No Hit
GTCATGTCTTGGACCCCTTGCCAGGCAGAGAGACCCAGGGCAGACGGAGG15.0No Hit
CCTGCCAGGATTGAATCCTAAAGAAACAGAAATATGAACAGACCAATGAG15.0No Hit
GAGTTTAATAAAGTTCCCTTCTCACCCTGCTGCATACTCAGAGCCTTTGC15.0No Hit
GGGCTTGGGAAGAGGGGGTGCCGCCACTCATCACCCCTACCCCACACCGT15.0No Hit
CTCTGAGGAAGGAGTCTGGGCAGAAGGAAGGCCTGTCCTGTGCCTCTGGT15.0No Hit
GATTTTATTGTGTTTATTTACAGAGGAAAAAAAAAAGAAAACCAGACTTA15.0No Hit
ATCAAAAGCTTTTTCTGCATCTGATGAGATGGTCCTATGATGTTGATCCT15.0No Hit
AGACGGTAGAGTGCATAGACAGCCATCCTAGGGCACGCACGCAGCTGCAG15.0No Hit
CTGCTCCCCTGACCCACTCTGCCCAGACGGGACAGCTTCCTCTGGACGCA15.0No Hit
AAAATAAAGTATTGGAACTATATCAAAATTTAAAAGTTCTGCACTTAGTA15.0No Hit
TTGTTAGCCTCACCTATCTGTGGAAGACATGAGGATGTGGGATTCTGATG15.0No Hit
TCATAACGGTTGCATATTTCTACAGCTCTTCTTTAAGGAGAAGCTGAGTG15.0No Hit
ATATTAGACTCAGAAGGACCTATAGGATCCAATTCGTATTTTTCCCTAAG15.0No Hit
GGTCCTTCATTGAATCATTTTGTCCAACAGTGAAATGCTAAATGACAGAA15.0No Hit
GCGAATGTCCCTCGAAGGAGCAGACCTATGCAATCTGCCAACTGAGCTAT15.0No Hit
CTTTCTGTCTCTACAATAAATAAATATGTCCTTAAAAATCTTATCCTGAA15.0No Hit
ACTCTACACTGTGCTAATGGCGATTCAGAATTATGTCAATCCCTAAAAAA15.0No Hit
GGGGGGGGGGGCTGGGGGGGGGGGCGGGGCGGGCGGCGGGGGGCGGGGCG15.0No Hit
GGACTCATCATCAGAAATGAAAGAGATGTTCCCTGAAACCACTGACATGC15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers