FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1985_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1985_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCTACACCCATGAAACCATTTTCTGAGCGTCCTGCTAAGCCAGTTTA10.6060606060606061No Hit
CATTTAGGAAGTGGATTCTGCCTCATGATAAGGGGAGCTTCGAAAAAATC10.6060606060606061No Hit
AAGCAGAACTGAGGGTAAGAGGGGTTCCTTTAACTTAATTTGTAGAGAGG10.6060606060606061No Hit
AATAAAAGTTCTGTGAATGTAGTCTCTCTCTCTCTCTTTCTCTTAAAATA10.6060606060606061No Hit
GCGCCGGCCCCGGGCTCCTGGGCCGTGGGGGGCCGCCGGGGGGGCGCATG10.6060606060606061No Hit
ATCCAAAAGAAAAGGATACATTTCTAGAAACATACAACCTGCCAAGATTG10.6060606060606061No Hit
GTTCAGAGTGGGTCGAACATGTCCAAACAAGAGCCCCTTACTGCCCCACT10.6060606060606061No Hit
TCTTTCTACACAGACTTCTTGATCGCTCGTGCAAAAACATCAGCATTGTC10.6060606060606061No Hit
CCAGCTGCAACATCTATAGCAGAGAAGAGTAGGTAACCATATGATATCGC10.6060606060606061No Hit
ATGTGTATGTGTGTGTACGCACGCCATTTCTTCTTTATCTCCTCTGTGGC10.6060606060606061No Hit
ATCTAAGGTGGAGAACGTTCTAGGGGCACTTGAGAGAAGTATATTCCATT10.6060606060606061No Hit
TCTCTCTTCTTCTGATGTTCTGACTTTTCTCACTTCAGAACCTTTGCACA10.6060606060606061No Hit
ATATTATAAAATACATATGTACAAGGAATTTTCAGAATTGGTTTATTCAG10.6060606060606061No Hit
CACCCAGAGTCAGAAAACAGTAAACACCCGCGTACCCCCCAACTCAGGAA10.6060606060606061No Hit
GAGCCACCATGGTGTCCTAAAAAATTAATTTTCTAAAATTGACCTCATGA10.6060606060606061No Hit
AAGTCATACAGAATGGCAGGCAGAGACAGAGGTAGAAGCAGGCTCCCTTC10.6060606060606061No Hit
TCTGCCTTCTGATCAGGTTATGATCTCAGAGTCCTTGTATCAAGTCATGA10.6060606060606061No Hit
TGACTGGAGTTCGTCCAGAAGTTTGCCTGCAGAGTCTCTCAGTGGAAGCA10.6060606060606061No Hit
ACCTTGCACTCAGTTTACATCAATGAAATGCATAGCTTTACATTCACTCC10.6060606060606061No Hit
GGTAAATGGTGGCAAATTCCTCATCTGTTCTTATAAGCAAAATTACTCTG10.6060606060606061No Hit
CAGGGGAGCCCACCTTCAAAGCCCACAGTTTCTCCCTCTCCGAACTGTGT10.6060606060606061No Hit
GCTTTAACCCACTGAGACACCCAGGTGCCCTGGGTTTCACCAGTTTTAAG10.6060606060606061No Hit
CAAAGGATATTAGCGACACAGCAGCTCACCACCTCATGGCTATATTTCAC10.6060606060606061No Hit
CTGTACTTTAAGAGGGATAGTTACAATCTAGAGGATTTTCAGGAGATATC10.6060606060606061No Hit
GAAGTGGGAATGCGGATGGACTTGGAGGCCACATGATGAAGTGACGCGGT10.6060606060606061No Hit
GAAATCAAGTGTCTGGGCTTTTCCTCTTCTGTAGTTGGACATAGCACCCC10.6060606060606061No Hit
GAGCCACCCAGGCACCCCATTTTTCGGCTGTTCTTGATTTCAACTCTCTC10.6060606060606061No Hit
GTGTGGCCCCTGGAAGGGGGCAATACTGTCATACCCATGTTCCTGGTGCT10.6060606060606061No Hit
GAGTAAATGCTTTCTCTGTGCTCGATACCTGGTCTCAGTTACGGAGGCCA10.6060606060606061No Hit
AGTAAATGGGAGTAAATGGTCGTCTGCCCACCTAGTTCCTCCCAGATGCC10.6060606060606061No Hit
GTGTTTGACGGCCATTAAAAATGATATTTAGTATGGGTTTTCGTGATGTG10.6060606060606061No Hit
TAGTCCTACCATACACCCGCCCAGCCAGAGCCGGTCTCAGGCTGATCTGA10.6060606060606061No Hit
GGTAATAGAATGTACATAGTTCTGTGTATGTGATTACATTACACAATACT10.6060606060606061No Hit
GACAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCATTTGCTGCTATT10.6060606060606061No Hit
GCCATGAACAAAGCACACCCCTGCTCTAAGTCTGGTTTTCTTTTTTTTTT10.6060606060606061No Hit
AAATAAGTGAAACCATATGATAACTGACTCTCTCTGCTTGACTTATTTCA10.6060606060606061No Hit
CCGAAGCCAAAGGCAGCGGCTTAATGCACTGCGCCCCCCAGGCGCCCCGC10.6060606060606061No Hit
CTTCCCTCATCACATCCAACATACATTCTGCTTGGAGGTAGGCCCTGCTC10.6060606060606061No Hit
TCCCAGGACCCTGAGACCATGACTTGAGGTTATCGCAGATGCTTAACTGA10.6060606060606061No Hit
CTCCAGGCCCCCACCAGTGCGGCTGATGCTGGTGTACCCGCAGAAAGCCC10.6060606060606061No Hit
AGGAAGGCCTGTCCTGTGCCTCTGGTCCCAAGGGGCAGCAGCTCCTTGGC10.6060606060606061No Hit
TCTCTAGCGTATGCTTGGGCACTCTCTGGCGCTGCAATATTGGAGTATGT10.6060606060606061No Hit
GTGGCTGGGCCATGGTTCTGGAGAATAGGGATGCCATCCACGTGGCCACA10.6060606060606061No Hit
CTCTTAAGACTTTCTGTGAGAGGGAAGTAGGAGAAGGGGAAATAAGCATT10.6060606060606061No Hit
TCCTAAGGAAGGAACACACCACAACTGTCACATGGGTGTACTGTCATAGC10.6060606060606061No Hit
CCCTTACGGAAACAGCCAGAATGGTGTTTGACTAAATTTCAGGACCCCTC10.6060606060606061No Hit
GCGCTATCAAATGCTTTTTCTGAATCTGTTGACATGGTCCTATGATGTTG10.6060606060606061No Hit
GTATCTCCTAGTTTAGAAAGCACTTTTATACACATCCTCGTATTGGAGTC10.6060606060606061No Hit
GGCTTGGTCACAGCACCGCCACCGCCCAGCACAGAACCCCGCACAAAGTA10.6060606060606061No Hit
GTCCCAGAGCTCCTGAGTCACAGCTGATGTGTGCCTTCTGCCAGGATGGC10.6060606060606061No Hit
GCCTTAAACCATGAGCTTGTCCAGTTCTCTTCTTGGGTTGAGTATAGATA10.6060606060606061No Hit
GTGATCTTCTGTCTGGATGGGGGATCATTGAGAGAAGTGTGAGGTATTAA10.6060606060606061No Hit
TCATTGGTCTGTTCATATTTCTGTTTCTTTAGGATTCAATATTGGCAGGT10.6060606060606061No Hit
CCTTAACCTTATACAGTGATATATGTCAATTAGTTCTCAATGAAATTGAG10.6060606060606061No Hit
CCTTTGCTGTGGAGGAGCCGCCCCAATTGACCGAATGCCTCCGGGAGCAA10.6060606060606061No Hit
GCTCCCTGCTCAGCAGAGTGCCCAATGCGGGGCTCGATCCCAGGACGCCG10.6060606060606061No Hit
CTGCTCAGCAGGGAGCCTGCTTCCCTCTTTCTCTCTCTCTCTGCCTGCCT10.6060606060606061No Hit
CTGCATCCTTCTAATGCCTTGCTTCTGTACTCCCTCCTTTAGGAGGCAGC10.6060606060606061No Hit
GGGTGGCTCAGTTGGTTAAGCAGCTGCCTTCGGCTCAGGTCATGATCCCA10.6060606060606061No Hit
TACCAAATCTAAGCTGTCCAGTCACAGCCCTGGGAACCCACACCTGGCAG10.6060606060606061No Hit
CCCCACGCGTGCGTCCCTCCGTGTGGCCGCGGTACCCCAAGAACCAGAGC10.6060606060606061No Hit
GGTCATACACCATCTGTTTTTGTTAAGCTGGTTGCATTATGAATCACCCA10.6060606060606061No Hit
GTCTTGTCCCAATAATGTTACTTATCGGCCCACGCCCATATGCATCTCGC10.6060606060606061No Hit
CACTCAACCGATTGAGCCCCCCAGGTTCCCGGGAGGTTTCAGTTTCTTAC10.6060606060606061No Hit
GCACTTAATTATCCTTGGGTCAATTAGATATAACTCTTTTACAAATTGAT10.6060606060606061No Hit
CAAGGGAGTGGTAAGGGCACTGGGGGTGGCACAGCTGGGCATCAGTTGCA10.6060606060606061No Hit
GGGATATTATAAATTGGTGAACGGCATGACAGTAAGAAGCTATCATCCCA10.6060606060606061No Hit
GGTACGTATTTATAAGGAAGTGATTGTTCCTATTAGCTAATGTTTTATTT10.6060606060606061No Hit
GCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGAACACCCA10.6060606060606061No Hit
TTCCACAGTCACACCTCCGCTCACACCTCCGCACTGCAGCGGTGCTGCTG10.6060606060606061No Hit
AGGCAGCGGCTCAAACCACTGAGCCACCCAGGCGCACGGTGTCACCATTT10.6060606060606061No Hit
GCGCGGGAGCGCGCGCGCGCATAGGAGGACCGCATGGCAGGCCGCGGCAG10.6060606060606061No Hit
ACCCCACCCTGGGCATAGAGCGCACTTTATTTTTTTGAAGAGGTCATTTA10.6060606060606061No Hit
GTCTTGGCTCATGAGGACTCCAAAGGGGTGAGGCTGGCCAGGTATAGATT10.6060606060606061No Hit
CCTCTCTCCCCCCAGCGAGGGTTTGAGCAAAAACTCTCCAGAGCACTTGG10.6060606060606061No Hit
TCAGGGAAGACCAGACCGGAGAGCCTGAGTCTCATCGGGAAGACAGCCGC10.6060606060606061No Hit
GTCTGGATGGGGGATCATTGAGGGAAGTGTGAGGTATTAAAGTTCCCTAT10.6060606060606061No Hit
GTCACACTGGGGTCTATCTATATAATACTATCTATATAATCCTTGTCACT10.6060606060606061No Hit
GTACTTGACCATTCCCTTAATTCCCCACAGCACAGCTGAGATCAGGTTGG10.6060606060606061No Hit
ATCCTAGATGGCTCCCCAGCCAGGAGCATCTTCTCCATCAAAGGGGTGGA10.6060606060606061No Hit
GTCTGAAAACAACTGAATTGTGGGATCTCTAAGGACCCTTCCAGATGGAC10.6060606060606061No Hit
GTACTAGATGACCTAGCAACAGCAATCAGAGAACAAAGCGAAATAGAAGG10.6060606060606061No Hit
GCCTGCTTCTGCACGTGCGCTGCCTCTCTCTCTGATAAATAAATGAAATC10.6060606060606061No Hit
TTATATCACATAATGACAATAATGTTATCAAAACTAAGAATATTTCCAAG10.6060606060606061No Hit
AAACCAGCTATGTATCACTTTGTTTCAAATTCTTTTCATTTGCAGTAGTA10.6060606060606061No Hit
GCCCAGCCCTGAATCATGTGTGCTATAGACGCAGCCTCCCCTGCAAGCCG10.6060606060606061No Hit
CAGGGCGCGTGCTCCACTCTTAGGAAGGAGTCTGGGTAGAAAGCATGCCT10.6060606060606061No Hit
CACACACACCCCTTGTGTAATGGCTTCTCCTGCTTCTGAGAGCGCTCTGC10.6060606060606061No Hit
CTGTGGGATTGTGGGCTTAGAGCTTCCACCCCGATTTCAGAAGGGCGGCT10.6060606060606061No Hit
GCGTATCAGGAGGCCGGGGATCAGCGAGTGTCTCTCCATGCGTCCGCCCC10.6060606060606061No Hit
GCATCTTCCTCTCCCAGGCTATTAGTCCTTTTGACAGCAAAGGACAATAA10.6060606060606061No Hit
GGGTTCTCTGCACAACAGAGAGCCTCCTTTTCCTAATCTCTCTCTCTGGA10.6060606060606061No Hit
GCTGTACCTATGCTAAGGCTAAACCTGAGGTGGGATGGCCTTCATTTTAC10.6060606060606061No Hit
GTTCAGGGTCGGCCCTTCAGGTGGCTTGCTGGGATTCGTGTGGGTGTGTG10.6060606060606061No Hit
CCCCGCGGGCACCCTACTGTGGCCTCCACGCTTGGGGCGCTGCTGCCGTG10.6060606060606061No Hit
GATCTGTACACCCCAGAGGCCAGTTTTTAGAAATACTGGTAGGCAAATAC10.6060606060606061No Hit
CTGCCTACTTCTGATCTCTGTCTGCAAATAAATAAATCTTTAAAAAAAAA10.6060606060606061No Hit
AACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAA10.6060606060606061No Hit
CTCCTGACTCACTGAAGACACAGTTCGGAGAGGGAGAAACTGTGGGCTTT10.6060606060606061No Hit
CATACTACTCTTGCCCTCATTTGTGGACGTGCACAAAGTGGTGAGAAATT10.6060606060606061No Hit
GGGTTGAGCCGCAGCCTTCGGCTCAGGTCATGATCTCAGGGTCCTGGGAT10.6060606060606061No Hit
GTAGGGACATACGCTCAGTGTGAAAGTTGACACCATAAGGCTTCTTAAAG10.6060606060606061No Hit
GTCCGCGCGTGTGCGCGGGTGTGCGCCTGTGCCCGCTGGGGTCGGGGGGG10.6060606060606061No Hit
ATTCACACTAAGCTGCTAATGACTGTAATCCTTTCTGGAAGGTTTGGGAA10.6060606060606061No Hit
ATCTAATCTGTCCCTCCCCCTACTCCCTTAAGCCCGGCGCCATCATCCCC10.6060606060606061No Hit
CCAAAAACCAACCCCCCCCACACAACACCCAACCCAAAACCACCAAAAAT10.6060606060606061No Hit
CATCTGCCCCGTGATTTAGGAGCCCCTGGGGACAGCAGCCCACGGGCTGT10.6060606060606061No Hit
GTCCAAGGCCAGGTAGTATGTAGGCCATTTCCCAGTAGTGTGCCCCCACT10.6060606060606061No Hit
GTGTGAGGAAGGGGACAGGGTGGACCCCAAGGTCCCTGGCCCTCCAAGGC10.6060606060606061No Hit
GAAACAGAAATATGCACCGACCAATGAGCAGTAGAGAGATTAAATAAGAC10.6060606060606061No Hit
CCTCTATTCTTCACAGCCGCCTCCCTGGAGGGACGTACTTCATCAGGAGC10.6060606060606061No Hit
GGTTAAGGGGGGAGAGAGAGAGAGCACAAGCAGAGGTAGGAGCAGAGGGA10.6060606060606061No Hit
TTACCCACCCAGAAAGGAGCAACTAAAAATGTAGAACATAAATGAACAAA10.6060606060606061No Hit
GGGTGGCACAGTCAGTTAGGCAGCTGACTCTTGATTTTGGCTCAAATCAA10.6060606060606061No Hit
TAGTCAGCCCGGGCGGGGGGGGGATTGGACTCTGGGAAATATCCATAGAA10.6060606060606061No Hit
GGGTGCACCCCCAGGGCCCTGAGTGCTAAGTGGGCACCATGCAATAGGCA10.6060606060606061No Hit
ATTGAAGGTTGAGTTATACCTGTGGGAACAAAAAAATGGTGGATGTTTTC10.6060606060606061No Hit
ACGTTGCTGTTTGGGCTCCAGTGGCCCTGGGTAAGCTCTTGGCTTCTTCC10.6060606060606061No Hit
GTCCAACACAGACATCACGCAGGAGCATGGGGATGGGGAGAATACTCAAA10.6060606060606061No Hit
GACCTGAGCCACGGGCAGATGCTTAACAACTGAGCCACCCAAGCACCCCT10.6060606060606061No Hit
CACCAGAGGGGCTTCTGAGCATCGGTGCGGGTGCAGGTGCCCGGGTTCCC10.6060606060606061No Hit
GGTGACTTCCTTCCCTTTTTCTTCACCTCGCTGGCTGGGTTTGTATATCC10.6060606060606061No Hit
AATTACACCCAAACACTCTACAAAAACAAAAAAAAAAAAAACCAAAACCA10.6060606060606061No Hit
CACCTGGGGAGTCATACCCACCATCCTCATAACGAGTATCTAACTTCGTG10.6060606060606061No Hit
GTGTAGACTGGGGAGCTAGCATTGAGCACAATAAAGCAGTAAAAATAGTT10.6060606060606061No Hit
GTGCAGAGTGGAACAGTGGCTAAGAACACGGGTTTTCAAAGTCGCCCACC10.6060606060606061No Hit
GCTCCATAGTATTGCATGACGAAAACGTCAGACTACAGTTGCAAGACCTC10.6060606060606061No Hit
GGCAGAGAGAGAGGGAGAAACAGGCTCCCTGCCGAGCAGCGAGCCCGATG10.6060606060606061No Hit
CCCATAAAGCATTAGCAGATCAAGAAAAGCAAACACAAAACTAATATTAA10.6060606060606061No Hit
CCTGTGCACTTATCACTGCGAGCCGTTTGCCCCGTGTGTTGGGGGACAGT10.6060606060606061No Hit
GCCGTGATAAAACCCACAGAGCCTTCAGTGAAATTTACCCTGTCTAATGA10.6060606060606061No Hit
GAATCCCACCAGGACTCCCAAGCAGACCAAGCAGTGGGAGCTGGCCTGAA10.6060606060606061No Hit
GGGCTAGGGGCCGGGCGGGAGTGGGCCTCGGGCGGGGGGGCCCGGGGGGC10.6060606060606061No Hit
CATCAGGCTCTCTGCTCAGTGGGGAGGCTGCTTCCCCCTCTCTCTGCCTG10.6060606060606061No Hit
GTATTGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATTT10.6060606060606061No Hit
CCCTGCAATTGCACTACTGGGTATTTACCCCAAAGATACAGATGTAGTGA10.6060606060606061No Hit
AGCGAAAGCAGGGGAAAAAAAGGTACAAGATGTTTATCCTGACCTAGATG10.6060606060606061No Hit
GACAAATACCGTATGATTTCACTTATATGTGGAATCTAAAAAACAAAACA10.6060606060606061No Hit
TTCCGCGGGCAGGGGACCTGTGCCATTACTCAGGGGCCCACCCACAGAAA10.6060606060606061No Hit
CCCCAGAGCAGGGACTCCAACGTGGGGATCGATCCCCGGGCCCTGGGGTG10.6060606060606061No Hit
CAAACCTAAGAGAAGCAATCAGCACCAATACCATAGGGAACTTTAATACC10.6060606060606061No Hit
CTGCCGGTGTGGCTTGGTCTGTTAGGTGTTGGCCTTTGGCTCAAGTCATG10.6060606060606061No Hit
CACTTAGCACTCAGGGCCCTGGGGGTGCACCCTGTCCCAAGGGGGGCTTG10.6060606060606061No Hit
GAAAGAGAGAGCACAACCCGGGGGAGCGGGAGGCAGAGGGAGAAGCAGTT10.6060606060606061No Hit
CACCATATCTGGAGACTGACTGCTTGGGTACCAGCCACCCCATTGTCACT10.6060606060606061No Hit
GAGTAAACACAGTAATCCCCCCTTATCCATGGTTTTGCTCACAACAGTTT10.6060606060606061No Hit
CTCCTATGAAGATGATAGGTCGGTTTGTGAGACCGGGCCTTTAGTTTCAT10.6060606060606061No Hit
TTCTGGGAGTATAGCCCAAAAGTGGCCGCGGCGGGTCGCGGGGAGCTAGG10.6060606060606061No Hit
CTTTAATACCTCACACTTCCCTCAATGATCCCCCATCCAGACAGAAGATC10.6060606060606061No Hit
GTTTTGTTGTGCTCATTTTAAAATTGGTTTGTCTTCTTGTTAATGATTCA10.6060606060606061No Hit
GTATATAACCCTACCGTTCTTCTCCTTTCTATCTAACAAGGTTCGCCGCA10.6060606060606061No Hit
GTGGAGGACAGGAAAAGATAGGCAGGCTGTCAGGCTTGAACACGCAGCAG10.6060606060606061No Hit
CAGTGAGGCTGTGGGCTTACCCTGGGTGTCCTGTCTGCCGCCCAGCTGCC10.6060606060606061No Hit
AAGCATTAGCAGATGAAGATAAGCAAAAACAACCCTAATTTTAAACCTAT10.6060606060606061No Hit
CCACATAGGTTCATATCACCACTGAATTCCTCAGAAAAGCCTTCAGCTAC10.6060606060606061No Hit
CTCTTGGAGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCCTTCGGCTC10.6060606060606061No Hit
ACTGGGCACCGAGAGGAGAGCCGGGGCACCGAGGACCCCAGCTTTGTTTC10.6060606060606061No Hit
TACTAAATCCAATTCTCATGGCAGAAAGCGTCTAGAACATCACCTTGCGC10.6060606060606061No Hit
CACTGGAATATTATGACTTCAAGTGATTTTATTTGGATATGGGGACCTTT10.6060606060606061No Hit
AAGTTAGGTTGCTTATTTGAGATGTTTCTTGTTTCTTGTGTTAGGCCTGT10.6060606060606061No Hit
AGCCAGGGGCCCTGGACTTAATGCAGTGTCCAGCACATAGCCAGTGTTAA10.6060606060606061No Hit
GCTCAGCAATGGTTCACTGACTCCCTGCCAACCACCATTGTTGGGGCTCT10.6060606060606061No Hit
GCTGCGCGGCCTCGGGCGGGCGGGCGGCAGTGGCCGCCGCGGGGCCCGGT10.6060606060606061No Hit
GAACCCTGCACATGGCACATGATCAAAAGATGTTCGGGAAATGAGGGGCT10.6060606060606061No Hit
CTTATACACTAACAATGAAAATACAGAAAGGTAAATTAGAGAATTGATTC10.6060606060606061No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers