FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1985-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1985-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105202
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC11800.10676781258086757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3650.047.686171
GCGAAAG4050.042.9667052
CGAAAGC4850.035.877793
GTATTAT9350.034.903981
TATACTG5300.027.3583985
GAATAAC5750.026.4865591
TATTATC7950.024.6247842
GTCCTAT9450.023.790521
TACACCG1650.002601083421.9696225
GGGTCGA3901.2198143E-820.4486489
TATAATA6051.8189894E-1219.1752242
CCAAACG1950.00684113818.58968145
TCCTATG11050.018.3726392
CTTAAGC3953.1220407E-718.3551983
TCTAATA7200.018.1265772
GTATTAA14050.018.0661561
ATTATAC4054.0588748E-717.9019833
TAATACA14500.017.5007324
CCTATAC2901.8649989E-417.500733
ATAACAG10000.017.4007283
Produced by FastQC (version 0.11.5)