FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1985-d10-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1985-d10-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697255
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG7090.10168446264279209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC902.8194336E-1064.46233
AGCGAAA3150.046.061021
GTGATCG1151.4118632E-744.1299979
CGAAAGC3700.041.160053
GCGAAAG3600.040.2947162
TCTAGTA2202.2530003E-829.6715642
GTACTGT2951.4551915E-1129.4912346
CCTATGC2654.5401976E-927.3660723
CTTAACG1409.954548E-425.909321
TATACAT3501.2914825E-1024.8568955
TAGTACT2051.2889181E-524.7576264
CTATGCG1801.5190043E-424.1681614
GTATTAC2451.9001618E-623.6885241
GTATTAG2151.8555342E-523.6196611
GTATAAG1851.822024E-423.5284671
GTATTAT6650.022.9092961
TATGCGG1952.6138188E-422.307475
GAGTGAT3055.335896E-721.393237
GTAATAG1750.00364418620.7274591
GTCCTAC2806.0525163E-620.7274571
Produced by FastQC (version 0.11.5)