FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1985-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1985-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396218
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC25290.18113217276958182No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG17160.12290344344507806No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT14050.10062898487198992No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG1352.1365926E-532.235911
TATCGTG950.004727222530.527255
TATACGT1504.395799E-529.0040042
ATAAGCG1000.006074361529.000895
TATACTG6600.027.4629635
ATGTTGT20300.025.7141536
CATGTTG16800.024.5989695
GAATAAC8350.024.3217011
GTATTAG3902.0008883E-1124.176931
GCATGTT22950.023.6935374
TAGCATG23750.022.5925922
GTATTAT10500.022.1046221
GTAGCAT25700.022.0132371
GCTACTA5950.021.932669
TATAAGA4307.094059E-1121.921632
GAGTATA16900.021.020601145
ACCGATC2505.9769103E-520.2998948
TTGTTTA25400.019.9802119
GTACTGC2959.567102E-619.6609136
GTATAGG3401.5095447E-619.1993271
Produced by FastQC (version 0.11.5)