FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1985-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1985-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925092
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12700.13728364314035793No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9490.10258439160645642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTA2101.4388206E-831.072964
AGGTCGG3950.027.5315138
GTTACGT2156.106693E-726.9853651
GTTGACA9100.026.2996121
CCTATAT2259.0809226E-725.7860151
GCGAAAG3408.913048E-1125.590882
TATACTG4250.025.5894955
GGTCCTA12700.024.5471259
CATTCTA8350.024.313892
ACATGGT10700.023.7161185
CGAAAGC4053.274181E-1123.2740083
AGCGAAA3753.0559022E-1023.2074151
TACGTGT2852.7755596E-722.89713
GATATAC2903.282239E-722.5023233
TATTAGA3554.118192E-922.4671082
GGTCGGG5850.022.3075859
CAGGTCG4951.8189894E-1221.969597
CTACATC5400.021.48373
GTAATAT4151.0877557E-920.9705561
CTACTGA13200.020.872246
Produced by FastQC (version 0.11.5)