FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1985-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1985-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755254
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC9810.1298900767159128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG1406.6068606E-736.2499353
ATACGTG2003.2526077E-728.9999475
TACGGGT1050.00770734427.6194
GTATACC1409.992424E-425.892813
GGGTTGA9900.025.6312666
TACACCG1701.03097846E-425.5881885
GTATAAG4850.023.9238191
GGACTGT6350.022.8346046
ACACCTA5500.022.4149861
GGTTGAA11050.022.3076527
GTGTGGG18950.022.195811
TCTACGG1650.002600303921.9696562
CCAAGCG1650.002600303921.9696569
TAGACAG6400.021.5233975
GCTATAC2404.3611282E-521.1457942
GTAATAC2505.959942E-520.305341
TATACGT2505.9721035E-520.2999634
AGTATAC2155.0546223E-420.2325212
GAGTAGG4001.6250851E-819.9427431
TAGATAG3301.1372449E-619.7726925
Produced by FastQC (version 0.11.5)