FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1984-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1984-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732333
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG2550.036.9656752
CGAAAGC2750.034.274923
AGCGAAA2603.6379788E-1233.475111
TATACTG2850.033.0700345
GTCTATA1601.8702794E-631.7316171
GTGATCG1601.8762712E-631.7186159
TAGTCGG950.004726575730.5261848
GTATTAT5450.025.285481
CCGACTA1450.001226160124.9998934
ATATTAG2101.549456E-524.176471
TATATCA2003.1020306E-421.7499074
TAGTACC2404.3609743E-521.1457444
ACTATAC3106.2395156E-721.0482988
AGTACCT3106.2395156E-721.0482985
GTATATA3808.991265E-920.9953541
CTAATAC1750.003656451620.7156123
GTTGACA5650.020.53931
ATCTAAT2155.0405576E-420.2407631
TTAGAGT3601.1750308E-720.1388034
GTAGGAC3651.3579847E-719.8642863
Produced by FastQC (version 0.11.5)