FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1984-d10-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1984-d10-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538744
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG6120.11359755282657441No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTACG450.00961949848.3552472
GGATAGC3200.036.2496035
AAGCGGT1854.1745807E-935.269896
TATGGAT3500.033.1578832
TGGATAG3750.030.938744
CACCGTA950.004725632730.5259849
GTATGGA4500.029.0158441
ATAGCAA4000.028.9996837
ACGCTAA2054.0140912E-728.3081381
GCTAATA1804.6747664E-628.2098461
GTATAAG1306.4583746E-427.8998491
GATAGCA4250.027.293826
CGGTCCA2405.2627E-827.1872049
ATAGTAT1100.00965072926.373143
AGCGGTC2808.179995E-925.8925767
AGCACCG2601.1428165E-725.0958825
GGATATC1751.2500296E-424.8707221
GGTACTG2351.3286608E-624.6805825
ATGGATA5000.024.6588863
GTATAAA3251.4715624E-924.5518671
Produced by FastQC (version 0.11.5)