FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1984-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1984-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194959
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC4100.2103006273113834No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC2090.10720202709287593No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAT502.0037232E-458.0145721
TATACTG1004.6773494E-850.7497335
CGGAGCG657.3221937E-444.61515145
GAGCAAC1053.8083926E-641.428356
GCAACTA1105.242695E-639.5452468
TCTATAC2600.039.048273
TAGGTGT1001.3873879E-436.249814
ATAAGGT850.002733229234.126223
GTAGTGC850.002736670434.1174668
GTTCTAT3250.033.4699441
CTATACC3350.032.4625134
GAGTATA2705.456968E-1232.22205145
AACCTAC2051.1286829E-831.8290986
ACCTTAT950.004712269630.5339831
TAAGTTC1000.006062787528.9998475
ATACTGT1255.138978E-428.9998476
GAGACTT1804.6747173E-628.1942949
CAACTAA1555.478262E-528.0643679
TATACCA3650.027.808075
AAGAGAC2156.082446E-726.9766037
Produced by FastQC (version 0.11.5)