FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1983_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1983_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACCCAGGACTAACACACGGGTGGGTCCTGCGATCAGGGCCCTGCGCAT10.6493506493506493No Hit
GTGGTGACTGGCAGGCGGCTTTTTTTTTTTCTGGTGCAGGAGGAGGAATC10.6493506493506493No Hit
GCACAGCAGGAGGCCACGTCCCTCACACAAAACGACAGAAGCCCCAGTGT10.6493506493506493No Hit
GTTCTGATCAGCTCGGTACTGCTCAGTCAGGGCCAAGGCACTGAGGTGAT10.6493506493506493No Hit
ATCTAAGAAGGTAAGTCCAGGAAGGCAGAGATTTCTGTTTGCTTTGTTAA10.6493506493506493No Hit
CTTCAAATGAGGTTCCTGGAGTGGGCCCTAATGCAAGACGGCTGGTGTAC10.6493506493506493No Hit
CTGCTGGGGCATCCCCTCTGGGATAGAACATCAGTCTTTGGGATATCAGA10.6493506493506493No Hit
GTGGAAGGAGCAGAATGCATTATAAGAGAGAGAAAGGGGCTAACAGTGCT10.6493506493506493No Hit
GTGAAAGAGGATAAGATATTCCCGGGGAAGGGCTCCTGATAGTGTGGAGT10.6493506493506493No Hit
ATGGACGCCTAGCATTTTACGGGAAAGGGTCAAGGAGTGACGTGGGAGAG10.6493506493506493No Hit
GCTGTGTGCCCTGGCCCGGGCGCCTCGGCCCCAGGGGCGGGGGGGGTCTC10.6493506493506493No Hit
GGATAATGGGTGGTCATGTAGAAGGAAAGTGTATATAATCAGGTGAAACG10.6493506493506493No Hit
TCGGGGGACTCCACAGAACTGGGGGCACAGTCTGAAAACAACTGAATTGT10.6493506493506493No Hit
ATTTATGGAATATATGAAAAAAACTAAGATAATCAATCATCACAAATACA10.6493506493506493No Hit
TACTGAGACATTCAAGTGACAAGGCAATTTCACACTCAGCATTAGTGGGC10.6493506493506493No Hit
CTGTGTGTCGGGCCAGTCGCCGCCTGTGGGGCGCGGGAGGCTGGGGCCGC10.6493506493506493No Hit
GTCCTGCAGGCACTGGTGGCAGGGCTGAAGGCCTACAGTACCTGGGAGAA10.6493506493506493No Hit
AGCCTTGGTCGCAGATGGTTAGGGAGCTGCCCATGTCTTCTTTCTGCCAT10.6493506493506493No Hit
ATGTAGGACTGTCCCCTCCCCAAGCAGAATGAGAGACCTGTGCTGACACA10.6493506493506493No Hit
GGAGAAGAGAGTGACGCAAAGAGAATTGTGTCCAAGAATCACGAGATGCA10.6493506493506493No Hit
GACTTGACCCGGAAGGGCATTCCCAGCCCTGAGCGCGGTGTAGGCAAAGA10.6493506493506493No Hit
CGCATCAGCCAGTCGAAGGAACGGTGCTCGCCCCGGGGGCCCGGGGAGTG10.6493506493506493No Hit
GAACAGGATGATTTTCTCACGCTGTAAGTGGGGAGACCAAGCACTTGAGA10.6493506493506493No Hit
AACAACACAAAGAAACCGAAATATATAGTGTGTTCCATTACCAAAAGGGA10.6493506493506493No Hit
TTCCTTGTTGAGTGGTTGCGGTCGGGGGGCCCTGGGGCGGTGGGGTGGCG10.6493506493506493No Hit
GAATAGATCAAAATAACAAAAATATGTAAACATGTACGTATTGTGTTTGG10.6493506493506493No Hit
GTGTGTGGTTGTGTGTGTGTGTGTATGTACACAGTCCTCCAGTGGATGTG10.6493506493506493No Hit
TCCTTAAACATGTCCGCGTCCGACTTCCCGAATGGGTCTGCAGACGGCAA10.6493506493506493No Hit
ACACAGGAGGGATGTCACGTGGCCTCGGTGTCCAGTGGCCTCAGCGCCCG10.6493506493506493No Hit
GTGATGGCCTCATAAAATGAGGTGGGGAGCGTTCCTTCCTCTTGTTTTTC10.6493506493506493No Hit
GCATGAGGTTCTTGAGGCCCTGGAAGGTGTCGGGCTTGAAGACACCATCG10.6493506493506493No Hit
GGCACCTCTGGGGCCTGCAGAGCTCACCATGCCTCCTGTGCACTCGCGGC10.6493506493506493No Hit
ATGCCCAGGTTTCACCTGAGCCAGTACAAAAAATGGCAACCCCATTAAGG10.6493506493506493No Hit
GCCATTTATGTGGATCTGATATCAGGATATCCAGGTGCTAGGAAAAGCCC10.6493506493506493No Hit
GTGTTGGCCTTTGGCTGGGGTGGGGGTTTTAGGGGCGTGGGATTGCGCGG10.6493506493506493No Hit
GAGAGAGATACAGCAAGAGAGGGAACACAAGCAGGGAGAGCAGGAGGGGG10.6493506493506493No Hit
GAACAAGAATTCTCATTTTTTAAAAAAGTTATTTGAAAGAAATTTTCAAT10.6493506493506493No Hit
GGGCGAGTCCGCTCTTCCCTTCAGCATGGAGTAAGGGTGGCAGTAGGGAG10.6493506493506493No Hit
CCTCATCACAGTACCGCCCATCATTAGTTAAGGCTGTGGTGGCTGGTATT10.6493506493506493No Hit
TAATTCTACTAAATTTTTACTTTATTTGTTCTTTTATTTTTAATGTATGT10.6493506493506493No Hit
AATATATACTTTGTAAATATCATCTCCCAGTCTGTGGTTAGCTTTTTAAT10.6493506493506493No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG10.6493506493506493No Hit
CAATGAAGCTAGGTTGTTTCTGACGCTGCTGGAAAGAGTGAAAAGAACGT10.6493506493506493No Hit
GTTAACTAGCCACTGAAGACCGTCTCCCTAGCATAGCATGAGTCATTTGC10.6493506493506493No Hit
GGATATACATACCGCCTGGTGCCGTTCGTGCTTTGTGCGGCCTCAGGCGC10.6493506493506493No Hit
GCAGAGAGGTGAGGGAATGTGGCACATGCTACACGTTCCTGGTCCTTCAC10.6493506493506493No Hit
CATGAACCCACTTGGCATCTGCAGCGACCCAAGGGATGCCGTGTCGAAGT10.6493506493506493No Hit
GAATAGTACATCCAATGCTTTGACCCCCTCATTTCTATGACCTCTTTTTA10.6493506493506493No Hit
CCGGTGTGGGGAGGCGCTCTGTTCTCTCCGCGCGCTGCCCGGCGCACGGG10.6493506493506493No Hit
ACCTCTTCTTCCTGCATGTTCTGCGGTAGCCTTTCCAATACAGCTTTCTC10.6493506493506493No Hit
TTTCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCA10.6493506493506493No Hit
AAAGGGAACCAGGGCCTGCTCAGATTCTATCTCCCAGGGTTAAGTCACCA10.6493506493506493No Hit
ATACAGAGTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGTC10.6493506493506493No Hit
GGATTATAGCTTCATGTAGGCAATATGCAGCTAGATCAACACATGAATAC10.6493506493506493No Hit
AGGCAAGGGTGAGTGGGGACAGGAAAACGAAGGGTGTGTACTTACAAGGC10.6493506493506493No Hit
GGAGGGGAGAGAGCACAGAGGAGGAGAGGGGAGCAAGGAAAGGACAGAGA10.6493506493506493No Hit
GGGTTGGTCGGCCTCATGGGGGGGGGGTGCCCTCGTGGCGGCCGGGCGGG10.6493506493506493No Hit
TTGTTAGGTAGCTCTATTTTTAGCTTTTTGAGGAACCTCCACATTGTTCT10.6493506493506493No Hit
GTGACTCAGGAGCTCTGGGACTTGCTGGAGCAGACCCTTAGCCTCTCAAG10.6493506493506493No Hit
CTCCAGTAGCCCATGAGACAGTCCGGAAACTTATGGGCCCCGCCTACCCC10.6493506493506493No Hit
GTGTCGAAGTTCTCGGGCGCCGACTCTGCTCACACCAGCGGCCCAAAGTC10.6493506493506493No Hit
GTATTAATTTTTTTTTTCTGTTTATGATTAGTATAATTAACAGTTTAAAC10.6493506493506493No Hit
CCCATGTAGGGACAGTGGTGGTCGTGACGCCCCACCACTTCAAGGACCGC10.6493506493506493No Hit
GACCTTGCTTGGCCTTGGGAAGGCTGCACCCAAACATGTAATCGAGTTAA10.6493506493506493No Hit
GTGTGCGAGTGTTCCGGGGTCGGGTAGCGGGGGGGAGGGCGGCAGCCGTG10.6493506493506493No Hit
TCCATGACTCACAACTACCTACGACTTACCAGAGGAGAACCACCCCCCTA10.6493506493506493No Hit
ATCCAGAATAGATAACATCCTCAGTCATAAATCAGGACTCAACTGGTATC10.6493506493506493No Hit
GGCCCCGCGTAGCTTCCCCGGGCGCGTCGGGGGCGCGGGCCTGGCGGTGG10.6493506493506493No Hit
GAGTTACAGTCATTCTTTATGTTCTCTGGTTGCTAAACCTTTATTGGTTA10.6493506493506493No Hit
CATGGATCCCAGGATCCCCCACCTAGGCTGGTGGGAATTGGCGCTATTCC10.6493506493506493No Hit
AGCATGGACTTGGAGAGAGAATATGGTCTCTCAATTCAGATGAAATAACC10.6493506493506493No Hit
ATTCCTCCTTGTTGCTGCGGGGGATCCCCCCAGTGTTTGTCTCCCCTGGG10.6493506493506493No Hit
GTTCTCGTGTGCAAAAGTAGAGGGGCAGGTAGGAACCGGGACAGGAGCCC10.6493506493506493No Hit
ATATGGAAGTGGGGCAACAGTGGGACATTAGTGCAAAACTGAAAGGTAGC10.6493506493506493No Hit
ATTCAATACAGCTCTTCTCAAGCAGAAAAACAAGTATACCTTGAGAGTAT10.6493506493506493No Hit
GTCACCACCCGCGTAGGGTCACAGAGCTAGCGTCGCCGCACAAGGAAAAG10.6493506493506493No Hit
GTCCCGCTCCTTATCCGAATGTCCGCTGCCAGGCCTCAAGGTACCGCCTC10.6493506493506493No Hit
GAGCCACCCACGTGTCCCTGGAAGAGAGTTTTTAGAGCAGGTCAGTTAAG10.6493506493506493No Hit
GGTCTGGTCTCGAGGAGACTCATTTCCAGGTGACACCTGCCAGGTATGGC10.6493506493506493No Hit
ATACACATTCTCTTATTGGAGTCAGGATATGGTCATGGGAGAAGAATTTA10.6493506493506493No Hit
TTCCGGGAGGGCGGCGGGCCGCTCCTGGCACACGCACAGTGGCCCGCTAG10.6493506493506493No Hit
ATAATGAGGAGGCTTTAGAAAGGCTCTCAGAGTAGAAGGTATTTGAATTG10.6493506493506493No Hit
CACTGTAACAAGATCCAATGAAGGGCACTACAACTGATAATGGAATTAAT10.6493506493506493No Hit
CTGTGACTCCTGATCTTGTGGTTTTGAGTTTCAACCCCACACTGGGCATA10.6493506493506493No Hit
TGTTGAAGGAGTTCTTGGGGAGCCCCCGGTCTGACAGCCTGTTGTAGTTC10.6493506493506493No Hit
GACCTTGCCCAAGCCTCACTCCCACATCGCCGCCCTGGGGCTGGTACCTT10.6493506493506493No Hit
GGCAGGCAGGGACCTGCGGAAGAAGAGTTCTCCAAGAGTAGATATTTGTG10.6493506493506493No Hit
ATCTAAAAAGAGCAAATCAAATGCACTTCAACTGATAATGGAATTAATGA10.6493506493506493No Hit
GAGGGAAGGTGGAAGAGGGAAAGGGGAGAGAAAAGAGGAGAGAGGAATGG10.6493506493506493No Hit
GGGTGTCCCCTTGAGGTTTAGGGGTGGGGGAGGGTCTCGGTCCTTAGCAC10.6493506493506493No Hit
ATATTGCTGTGTAAATCAACAATCACTTCCCAATAATTAAAAGCCAAGTC10.6493506493506493No Hit
GCTCTGATGCTAATTTGAGCTGTTAATTTAGACAAGTTATTCACACTGTC10.6493506493506493No Hit
GACTAGGGACGCTCGCGGGAGGGGCCTCGGGCTCCGGGCCCCCGGGGGGG10.6493506493506493No Hit
GGTATAGCTCCAGGGGATCTTAACCAGCCTTTTGCTTATAGGGCATAAAG10.6493506493506493No Hit
GGATGGCACAGTGGGGTGGCAGTGGTACAGGGTGTGGCTACCAGCCAGGC10.6493506493506493No Hit
CCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCACTA10.6493506493506493No Hit
GCTTTGGTCAATGGAGAGGATCCCACCCCAGTCAGTCACTGACAGAGGCC10.6493506493506493No Hit
GTATTGGGCGCCATGCAGCTTGAATTTCTGGAATGAAATGGCATCAGCTG10.6493506493506493No Hit
AGCGAAAGCAGGGCACCCACCTGAAGGCTGGACTGTCACCAGGGTGGTCT10.6493506493506493No Hit
AACCTGGCCTGTTGCTTTCGATAATGGCCAGAGCTGTCCTCTACCTCAGC10.6493506493506493No Hit
CCAGAGGGGGAGAGACAAGGTCTGGGTCTGGCCATGCGAGAACAGGGGAT10.6493506493506493No Hit
GGATCATACATCTTCCCGTATTGTATAATTTGGTTTTCAGATATTTTGGT10.6493506493506493No Hit
CCCCCACACTCCTTACCTCACCGGTGTGCGCCCAGAGATGCCCTCAGAGC10.6493506493506493No Hit
ACAGCAGAGTCCGTCGCTGCACATGGCGTCGTGAGTCAGGGTGCATTTCT10.6493506493506493No Hit
CCCATAAAAAGGGCCTAGCAAGAACCTGAAAGTTTAGGACTCCTCACGCC10.6493506493506493No Hit
CTAAGAAGGTAAGTCCAGGAAGGCAGAGATTTCTGTTTGCTTTGTTCCCC10.6493506493506493No Hit
GTTGCCGATGTGAGCAATGGGGTCTCTGAGGAACTGGCAGAAAAGTAAGC10.6493506493506493No Hit
CAAGGAGACAGGAGGGGAGGATTGGGCAGGGGATCGTTCTCCACTCTGAG10.6493506493506493No Hit
GGATGATATGCTAAGTCTATTTTTAGTGTTGTAGGGAACGGCCACATTGC10.6493506493506493No Hit
ATGCCTGAGAGATGGGTTTGATCCGCACTGTCCGACACACAGTAGCCACC10.6493506493506493No Hit
ATACTGAGATACTGGTTTTCACTCCTTACACGACAGATGATCCCAAAGCT10.6493506493506493No Hit
CTGTGCTGTGTAACAAATAACCTCCAAACTTGTCTGTGGCAAATAACCAT10.6493506493506493No Hit
AGCTGTGTACTCTTGGGCAAGTCAATTAACCTCTCTGAAACTCTCTTTCC10.6493506493506493No Hit
CACCAGGTGTCATCCATGTCAATGCTCCCCAACGCAAAAGACATGACTCA10.6493506493506493No Hit
GTTCATTCAATGGAGACAAACACTTTTTACATATCTCAATGTCTGGACAG10.6493506493506493No Hit
GTCTAAGGAAAATACTTGTAAAGGCCCTTGTAAAATAACAGAGCTCTGAC10.6493506493506493No Hit
ACTTTGAGGTAAATGAAAAAATAATACAATGAAACAAAATTCACAGAATG10.6493506493506493No Hit
GGCCCGGTCTTGGCACAAGAGTTGTCCATGATGGGCTCCAGGGGGGCCGT10.6493506493506493No Hit
GCCTTGAATGGCATGTCAGGTAGGCCAGGAAACGAGGTGAACAAACTCAC10.6493506493506493No Hit
GGCCTGAGGTGGATCAACCTGGATAACAACCGGATTCGCAAGATAGACCA10.6493506493506493No Hit
GCCCCAGGGCCAGGTGGCCAAGGAGCCGCTGTCTCCTTGGGCCCAGAGGC10.6493506493506493No Hit
GCAGAAATGTGCGCCTGTGCGCAGTGGGTTCTGAGAGGAATATGTTTTGA10.6493506493506493No Hit
CTTCCTTGTATTCTTAATTTTGGTCAAGATCTGAGAAAGAATACAGCAAA10.6493506493506493No Hit
TCCGTATACAGATTCTTTAACAAAGGTATAGCTCCAGGGGATCTTAACCA10.6493506493506493No Hit
AAGGATACATTTCTAGAAACATACAACATTCCAAGATTGAATCCTAAATA10.6493506493506493No Hit
GGTTATGTATACAAAGGACACTAATAGTAGTGGTGGGGAGCTGGGAGTCC10.6493506493506493No Hit
CATCATGGCCTGGACCCTCCCCAGGCCACCAGAGGTCCGATTCATCGGCT10.6493506493506493No Hit
ATCCTCACCCTAACACTAAACCCAAAGCCTGATCTAGAATTCTGACTAAA10.6493506493506493No Hit
GTTCAGGGATGCAGATGTAGTGAATGGGTGTAGAGGCGAGATGCGCAGGC10.6493506493506493No Hit
GCTGGGCCAGAGTGTATGACCTTTGGACTATGTGGCAAGAGGCTTCAGGG10.6493506493506493No Hit
GCGTACACACCTCCCATGAGCTGCCCTTCCTTAGTCCTAACACTCAGCAG10.6493506493506493No Hit
ACCCCGGGGTCATTTCGTGCAGGCTTCCCACGGGCGTCAACCACTGGCGA10.6493506493506493No Hit
GTCTTGGCAGGTGCCAGTGTCGGGGCCAGCAGGGAAGACTTGCCGGAGGC10.6493506493506493No Hit
GGTAGGAAGAGACTAGCAACATAGAAACTTAAAGAAAATCTTGGCCAAAG10.6493506493506493No Hit
AGACCCAACAGCTGCCCCCTCAGTCTTTACTGCATGCTACCTGTGTTTGG10.6493506493506493No Hit
GATCATCTGCTGAACATTTGGATTTTCAGGCAATGATTAGGCACTAGAAC10.6493506493506493No Hit
CCCCTGAAGATACAGACCCACAGAACCAAGCCACAAAACTGGACTCAGTG10.6493506493506493No Hit
GCGCAAGTGTGCGCGGGGGCGGGCGGGGGGCGGGGCTGCGTTTGTAGTGC10.6493506493506493No Hit
GGCAAGTACAATCCACTCAGAAATAAACAAAATTATGTAAGTACACAACT10.6493506493506493No Hit
GATTATCCTGGTTACTAGAAAAGAGACTGTTGGGAGTGTTACTGATCCAT10.6493506493506493No Hit
CGGGGGGGGGGGGCGGTTTCGGGGGGTGGGGGGGCGGGGGCCCGGGGGGG10.6493506493506493No Hit
GTCCTGAGCCAAAGGCAGAAGCTTCAATGACGGCACCGCCCCAGTGCCCC10.6493506493506493No Hit
CTATTAAGGACTTTTGCACTAGGCTGTCTCAGGGGGTGCTGAGTTGCTTA10.6493506493506493No Hit
GTGCACACGCGTGTATACATGCACCCGCATACCATGCTCTGTTCTAGCTT10.6493506493506493No Hit
CAAATCAAGTGCACTCCTAAAACAAATTTTATATAAAAAAAAGATTTAAA10.6493506493506493No Hit
GTGTCACTCACTGGCCTTCCCACGTGATTCATCTTCCAGGACCACTCCTG10.6493506493506493No Hit
CAGTAAGTGGCCCCCATGCGTTTTCCAGCTCCATGGCCACGGAAACAGAC10.6493506493506493No Hit
GGGTCCGCGTGCCACGGCGGCCGGGGGGCCCGAGGGATGAGAGAGGCAGG10.6493506493506493No Hit
AGATTATCAGCTCCCAGAGAGGCGAGAGCCCTAGCTTAAGGTTCTCCTCT10.6493506493506493No Hit
TCACAATCTCTGTGTCAAATCAATAAATGACATCTTCAAAAAAATAAATT10.6493506493506493No Hit
GGACCTCCGCAAGCCCTGCTCTGGAAGTCAGGAGACCCCCCTGCCCAGGT10.6493506493506493No Hit
GTGTGTGAGGGGACCTTGGGATGAGTATGTGGTGACCTGTGCAAAAAGTT10.6493506493506493No Hit
GCTCTCCAGAAGAGAAGGGACAGGGTTGCGGTGACCTAGTGACGGGGGCT10.6493506493506493No Hit
CTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCGGCCCTGCCCCG10.6493506493506493No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers