FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1983_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1983_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCCAGGGTCACTGCCTTGGCATTTCAAACGGGAGCAGCGTCGGCCTCC10.9433962264150944No Hit
GGAAAATTATAGGCTTCTGGAATTTAATGACTTGACTTGTGGCCATACAT10.9433962264150944No Hit
ATATTCACTATCTATTCCTCTGGATATAAGATTTCTAACTGTGCGTCAAA10.9433962264150944No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCACAT10.9433962264150944No Hit
TCTATTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA10.9433962264150944No Hit
CCTTCTAATTATAGGTTTTAACACAAAGCACACAGTAGGTGTTCAACTAG10.9433962264150944No Hit
TATGTAGAGCATATATGAACAAACCTAAGAGAAGCAATCAGCACCAATAC10.9433962264150944No Hit
AGACTGACATTCAAGTCGGTCCCTGCCCCATCTAGAAGCATTTTCTTCCA10.9433962264150944No Hit
CCCTGGGATCATGACCTAAGCCCAAGGCTGACGGTTTATGACTGAGCCCC10.9433962264150944No Hit
TTGCAAGACAAGTGAGCTGGTATGACCAGGATGTGATCCCAGGGCTAGCA10.9433962264150944No Hit
CTTTTAAGGGGACCAAACTAACGCAGGGCGCCTACTTTTAGTATTACTGA10.9433962264150944No Hit
CCTTCGCTGCGGGCACCAAGAGCTGGGGATGCCATCTGCTCCGAAGTTGG10.9433962264150944No Hit
TGCCAGGGGCTCCCCTTAGTATTTAAAAGGGAAACTTGAGTGGAAATTAC10.9433962264150944No Hit
GGGCGGCGGGGGCGGGGGGGGGGCGGGGGGGGCGGGGGGGGCGGGGGCGG10.9433962264150944No Hit
CAGTGTAATAGGAGTTTTTTCTACTTTGGTTGGTGAGGGTAGCAACTTTA10.9433962264150944No Hit
CTTAAGACTGAGCCGCCCAGGCGCCCCAGAAAGGTGATATTATGTCCCCT10.9433962264150944No Hit
CTTAACCCCCTCTATTTTCTGCCTTTCTAGTCTTGATTCCTCTCTATACT10.9433962264150944No Hit
GGGCGAAGCGGCCTTTCGAGCGCGAGTAGAAGTAGTATATATAGGGAGGG10.9433962264150944No Hit
GCACACGGCAGTACAACAGGGAGAAAGGGGCTCCCTGTTCTGAACAGGAA10.9433962264150944No Hit
GGCCTCTACTTCTATGGAATGCCGAAAACGGTGAATTGGCACTGTCTTCC10.9433962264150944No Hit
CTTACACCCCAACAAGCTGGCTCATCCCAACGCAACGGCTACCTTTCCAG10.9433962264150944No Hit
CCTCTCAAGTGCCCCTAGAACGTTCTCCACCTTAGGTCATATGTAAGGCC10.9433962264150944No Hit
CATTTGTGTAGTCCAACAGGGATGTCAAAGCCCAATTACAATTAGAAGCT10.9433962264150944No Hit
GGGGTGGGCGGGTGGGTGGGGGGGGTGGGGGGGGGGGGGGGTGGGGGGGG10.9433962264150944No Hit
CTCATGCAGGCTCAATTACAGGAAACTAAATTCATTACTTGAGCCAAAGG10.9433962264150944No Hit
CACAGCCAGGCTTCCGGGTGTTGGAGAGAATCGGAGGTGGGAAGAGAAGC10.9433962264150944No Hit
GTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCATTAGTTTGAT10.9433962264150944No Hit
GGGAAACGAAACAAAACAAAACAGCCGAAACGGGGGAGAGGCTGGGAGGC10.9433962264150944No Hit
TAGAACAACTTTTTTTTTTTTTTTATAAAGTTATGTAAAAACGAAACAAA10.9433962264150944No Hit
AACTGGCACCAATGAACTGATTGATAAATGGTGGCTCTCATTACTATTCT10.9433962264150944No Hit
GTTCAAACTTGTCTGAAATGTTAAGGGCAAATCTGCCAGGGCTCCCTTCC10.9433962264150944No Hit
ACCCTTGCCTTCCTGAGTATAGCTCAGTTGGTTGTAATGGGTTCTCTCTT10.9433962264150944No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT10.9433962264150944No Hit
AACCTGGAAAGAAGCAGACACCTAAGTAAGGTCAAGGTTTAGGTGGACAA10.9433962264150944No Hit
CAATCAAGTCAACAAATACACAAATGGCAACCTTCCAAGTCTTCTACGTC10.9433962264150944No Hit
AAGTATTTCTGTGTGCCTATTTTTGTTTGCCCAAGAAACCAATTGGTTGT10.9433962264150944No Hit
TAAATGCACCAGTAGAGAAGGCTATGTATAATAATTATATGTAATGCAAC10.9433962264150944No Hit
CACCCACAGCGGCCCCCCGGGCGTGCCAGAGGGTTGCAGAGTGTGGTCTG10.9433962264150944No Hit
GGTGTTGTCATAAGAAGAAATTTGAACACAAACCCACACGCATAAAGCGA10.9433962264150944No Hit
GTTCAATAGATTCCTCGCCTGGGCACGCCACTTCCTCCCTCCCGGACCCC10.9433962264150944No Hit
ACCCAATTGGTTGTTCAGTAGCATGTTGTTTAGTCTCTGTGTTTATGAAT10.9433962264150944No Hit
CCACCACTGTGTGCCCCACCAGGAGCTGAGAAAAGGGCAGATGGGCCTCT10.9433962264150944No Hit
GTGATATACTTTGGAGCTACCATCACCCCATTGGGTCTTCTGTCTGTGAT10.9433962264150944No Hit
CTGAGTTCCTTTGGCCAACAGGACAATAGCAAATGTGATGTGGGCAGAGC10.9433962264150944No Hit
GACTAAAACATTTAGGGAAAACATAGCTGAGAAGTTAATGCTTTAGGTTT10.9433962264150944No Hit
GCCAAAGATGCTTGTAATAGAAATGCCAGGAGGACTTTGTTGGGCCTGGG10.9433962264150944No Hit
GTGCTTTCCAGATATTGTCTTTAGTATACTGGCCAAACAAATGGACACCC10.9433962264150944No Hit
GATCCCTGCTCACCTTCAGGGACCTTGTTAGTAATTTTTACTCACACAGC10.9433962264150944No Hit
GCACCACCTGCACACCATCGTCCCTGTGTCCTCAGAGGAAGGCTGTGTCT10.9433962264150944No Hit
GTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGTGGGAGGAAAT10.9433962264150944No Hit
GGATACAACGCAGGGACACCCAGATGGAAGGGTTTATCAGCAAGGGCTCT10.9433962264150944No Hit
GTGCTTGGAGGCTTCCTTGGTTTCTGTGTAACGGTGATGAGTCAGGAGTT10.9433962264150944No Hit
CAATAAACAACGTCGATTTTAAACAAATAGACACGAATTTAAGCCCAACC10.9433962264150944No Hit
GTCTACTAATGACCAGTAGAGAGCTTAAATAAGAAATCAAAAACTTCCAA10.9433962264150944No Hit
GGGAGGAAGAGAGAATCGCAAGCAGACTCAACACTAACACAGAGCCCAAT10.9433962264150944No Hit
GCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCATTACCTTC10.9433962264150944No Hit
GCCCTTCATTGGCTCTTGTTACAGAGTTTGGATTTTTTCTTGTTCTGCTC10.9433962264150944No Hit
CATTAATACTCTCTGTCTTTTTTATAGACTGCTTTTAAAGGTTGCCCCTC10.9433962264150944No Hit
TATATGAACAAACCTAAGAGAAGCAATCAGCACCAATACCATAGGGAACT10.9433962264150944No Hit
CCCTAGCTCTGGCTCCACTCTGGGAGGCCCCGGCCATCAGCTCCCCTGCG10.9433962264150944No Hit
TGATGCAACTTGGGGACTGCACCTGAACCTGGGGGCTGATTGCCAGGCCA10.9433962264150944No Hit
GTGCAGCCCATGCCAGTAGCCGCTCTTCTGTGCCTTTCCTGAGCTGTAGT10.9433962264150944No Hit
GGTGGTGATACTAACATAAGTAATTTGTGGACGTCGCTGAAATGAAATGT10.9433962264150944No Hit
CTATCTGAGGCCTTCCCTGTAAACCCCTCAGTACGCCACAGTGAGCCACT10.9433962264150944No Hit
TCCTAGCACTGTGGGTATGCTGGCTGCACGCCCAGCGAGGTGTGAGTCTG10.9433962264150944No Hit
CTCTTTGGTGATACCATCTGCGTATTCCTTATATACATCTGGTGCTTGAG10.9433962264150944No Hit
CCCCTTCAGGGAGTCCTTCTGACTGACCCCAGGAGTCTTTGATTCCCTTC10.9433962264150944No Hit
AGTCAGTAAAGTCCAGACGCCTATAATTTTCCCAAATGAGGACTGAAATC10.9433962264150944No Hit
GTATATTCCTTTTGCTGCTTTTGGATGGAGGGTTCTGTGTGTATCTGTCA10.9433962264150944No Hit
GGTAAGGGAACTGAGCGATGCGAGGACTCTTGTGATTAGAGTCACTGATT10.9433962264150944No Hit
ATTAAGGTGTGCCATTTACCCTCATTTCTGTTCCTGGTTTCCTCACTTCT10.9433962264150944No Hit
TGCCAGAAGCTCCCAGGAAATCAAACCTTCCCACCATAGCACCAGGCCCG10.9433962264150944No Hit
CATTTCCAGAAACATACAACCTACTAAGATTGAATTCTAAAGAAACAGAC10.9433962264150944No Hit
ATTGGAAAACTGATTCCACATACAGGAAGCTTAACATTCTTGGTAACAAT10.9433962264150944No Hit
GTTCTATACCACGGTGGTTGGAAAAGCTGGTTGATTTGAGTTCAGTCCTC10.9433962264150944No Hit
TCCACACGTAGCAGGAAGCCTTACACGGGTCATCATAACACGACATGAGC10.9433962264150944No Hit
AGGTTAGAATGGGGACGATGACAAGCCATGCATCTTTGCATCGCCTCAAA10.9433962264150944No Hit
TATCTAATGATATAACCTTCTACTGTGATCAAACATAATTGTATCGGAGT10.9433962264150944No Hit
CACAAGCACTTATTACACACCAGTTATCTCATTTAAGCCCCACAGTAACA10.9433962264150944No Hit
CCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAGTCCTTCC10.9433962264150944No Hit
GAAGTGGGAATGCGGATGGCCTTGGAGGCCACATGCTGAAGTGACGCGGT10.9433962264150944No Hit
CCTTCATCTGGGTCCTCTGTAAGCGCACCTGCGTCCTTCCCAAGTATTGT10.9433962264150944No Hit
TACCACACTTTAATACCTCACACTTCTCTCAATGATCCCCCATCCAGACA10.9433962264150944No Hit
GTAGATGGTGAGGGGCTTTTCCTAGCACCTGGATATCCTGATATCAGATC10.9433962264150944No Hit
CATATATTCTGAAGACGAGGTGAGAGTCTTAACCATTGCTGGAAGAGGAC10.9433962264150944No Hit
GTTATATACAGAATATTCCACCCTAAAAGAACAAAATACACATTGTTCTC10.9433962264150944No Hit
GAAGCAGGCTCCCCGCTGAGCAGAGAGCCCGATGTGGGACTCGATTCCAG10.9433962264150944No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT10.9433962264150944No Hit
GTGTTGATCTGTTTTTCTTGTTGGTGTTTTTATTTTTAATATATATCTTT10.9433962264150944No Hit
GTTAATGAGTTGACCTTTTAGAAAGCTCTTAGGTAATCTAAGGATAGGGG10.9433962264150944No Hit
GGATTATTCCAATGGATGACCAGGGACTGAGGCTATATTTTCCAGGGTGG10.9433962264150944No Hit
GCATTTTCCGTTCTTTCCAGGACCTCCGCACGCCCTGCTCTGGATGTCCG10.9433962264150944No Hit
TATATGCCCTACGCTGAACTTTTTACCCATGCAACTTCTCTATGATAGAA10.9433962264150944No Hit
ATGGTAATGGAACTAATTCGGATGATAAAGCGAGGCATTAATGACCGGAA10.9433962264150944No Hit
AGGCGGTCGTTCCTTGCGCTCTACAGGAGATGGAGTTGATATAGCGAGGT10.9433962264150944No Hit
TCTGAGCACCTGCCAGCACTCAGACCCATTGACTGATATCCCCAGAGGTA10.9433962264150944No Hit
CTTCTAGGACCATGAGAAACAAATGTATGTTTTTTAAGCCACCCATTCTA10.9433962264150944No Hit
CATTGGTATTCATTTTTGTCTGTGGATGATTTGTAGAATTAACTTGTGAA10.9433962264150944No Hit
CACCACCCCCAATAAGTAAGCCATCAAGTAGATCCAGACTCAGTTCTTGT10.9433962264150944No Hit
GCTTAGGACCCTATGGCTCATGTCTCCTAACATCCCTCTTCTAGGAACAG10.9433962264150944No Hit
GTTAAATATATTCCCACCCTCCTGAAACACATCTCTAGAACATTTTCCCC10.9433962264150944No Hit
CGTAAATAGAATGCACCACTGGAAATAAGCAACTACAACCTCAATAAAGG10.9433962264150944No Hit
AAGCCAGCTAGCGACTTTTTTTAAGGCCCTTGCACTAAGAATTGTTTTTA10.9433962264150944No Hit
GATACTGAGTTCACTAGTCTGGGGAAGCACAGGGCCATGGATACACTTTT10.9433962264150944No Hit
GTACAAAGAAAGGTGGAATGCAGGAAGCAGTTAGGAATTCATCTTTAACA10.9433962264150944No Hit
ACATCCTCGTATTGGAGTCAGGATATGCTCATGGGAGAAGAATTTATTGT10.9433962264150944No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers