FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1983-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1983-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634880
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC7230.1138797883064516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG750.001480706738.6787261
TAGATAG1301.6538706E-533.461435
ATAACGC1102.4351993E-432.9544379
GTCTAAT1453.4809906E-530.0093541
TAGTCGG1000.006072906328.9999075
ATTATAC1100.00966541926.3656273
TATATAG2901.187982E-825.0018862
GTATATA3902.0008883E-1124.1742041
GTTACAC2401.5954993E-624.168493
TATAAGA3052.0318112E-823.7704144
ACGACAA1600.002174322322.6561786
GTCTACA2252.6431951E-522.562591
GTCCTAT5300.021.8936181
ATAATAC2653.7738446E-621.8884453
TATAGTA2653.776424E-621.8867235
GTATTAT4501.3096724E-1020.9509751
TACACCG2104.3118375E-420.7142165
TACTATA1800.004303486620.140412
CTATACT3259.818414E-720.0768594
GGACTGT6900.019.9637036
Produced by FastQC (version 0.11.5)