FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1983-d10-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1983-d10-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494631
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG8120.16416277993089798No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT7350.14859561976503696No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC7200.14556305609636275No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA5140.10391584837990342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA901.3167319E-648.3624341
GTGATCG604.9427606E-448.33319
AGCGAAA1351.018816E-842.9888271
GCGAAAG1656.00121E-835.15492
CATAATA1956.9703674E-933.4647562
CTAATAC1301.6531534E-533.4613723
ATTATAC1352.1400987E-532.2220653
TATACTG1601.8746523E-631.7185965
TATAAGA2351.2587407E-930.8540342
GTGTAGC1000.006053699629.0174581
ACGAACC1504.3944165E-528.9998575
AGGTCGG3403.6379788E-1227.7204518
CGAAAGC2104.978083E-727.618913
GTACTGC1907.146142E-626.7103966
GTAATAT1100.00963863226.3795071
TGTTATA1100.009662420526.366172
TAACCTA2259.0923095E-725.7776535
GTATTAT5350.025.7631651
CAGGTCG3751.0913936E-1125.133217
CCACGAA1851.8289062E-423.51343
Produced by FastQC (version 0.11.5)