FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1983-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1983-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682255
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC14310.20974562297088334No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT8460.12400055697649706No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC100.0070828204145.051682
TCGTTAT400.00603803654.402361
TATACTG3600.048.3363885
TTACGGA951.0277213E-438.16314
GTAATAT2300.037.845121
TAATATA2500.037.713442
GCTACTA850.00274151434.1173029
GTTACGG900.003622403632.2313463
TTATACT2506.91216E-1131.9043544
GTATATC1153.1517417E-431.53761
CCGCGTG950.004726488630.5260099
TCCGTAC1050.00769612427.6268673
GCCGCGT1050.00770714427.6187698
CTTGTAC1100.00965613826.3711013
GTTATAC1100.00965613826.3711013
GTATTAT3607.2759576E-1226.1937271
ATATCAC2301.0995391E-625.224533
TAGGACT5500.025.0488744
GTCCTAT7550.024.9794481
TATAACC2051.2866558E-524.7631073
Produced by FastQC (version 0.11.5)