FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1983-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1983-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663925
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA19940.3003351282147833No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG16500.24852204691795005No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT15600.2349662989042437No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA14340.21598825168505478No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC10750.16191587905260385No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG9340.1406785404977972No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT7680.11567571638362766No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC7580.1141695221598825No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG7400.11145837255714124No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC7180.1081447452649019No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA7150.10769288699777836No Hit
TCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCACT6660.10031253530142711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTCG4000.045.3118449
GTAGAAA7850.045.2779922
CAGGTAC2800.038.850423
GTATTAT4000.038.0906331
TACTGAT3100.037.4216277
GTGATCG1209.559963E-636.249479
TAGAAAC10150.035.724533
AGCGAAA2950.034.4322131
GTATACT850.002738484934.124864
GTCGTTT4250.034.117158
CAGTATG4250.034.117159
AGTAGAA10500.033.858341
GACAGTA4550.033.4635737
GGTCGTT4350.033.3353587
GCGAAAG3300.032.9714472
TAATATA2705.456968E-1232.238752
AGGTCGT4500.032.2241826
GTTGACA5000.031.9235781
ATTATAC1153.1556512E-431.530783
ACAGTAT4400.031.3063628
Produced by FastQC (version 0.11.5)