FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1982_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1982_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4771
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC150.3143994969608049No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT130.2724795640326975No Hit
GTTCTGGACCCTCCCTTCTCTCCTCCAGGCCCAGGTTCCGGCTCCCGCTT120.2515195975686439No Hit
GCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCATTACCTTC120.2515195975686439No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA100.20959966464053656No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT90.1886396981764829No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG80.16767973171242925No Hit
CCTCAAGACCATGAGAAGTCCCTGCTGGCTCCCTTCCTGAAGGTTCTTCT80.16767973171242925No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA80.16767973171242925No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA80.16767973171242925No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG80.16767973171242925No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT80.16767973171242925No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT70.1467197652483756No Hit
GTTCATTGATGCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGG70.1467197652483756No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA70.1467197652483756No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC70.1467197652483756No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC70.1467197652483756No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT60.12575979878432195No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG60.12575979878432195No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT60.12575979878432195No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG60.12575979878432195No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT60.12575979878432195No Hit
CCACAGAGGTGATACACAAATTCAAACTAGAAGATCATTTGAATTGAAGA60.12575979878432195No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG60.12575979878432195No Hit
GGTCACAACACTAAGCATGACGGATGACCCCTTCCCCTCTATCTTCCTAA60.12575979878432195No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA50.10479983232026828No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG50.10479983232026828No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG50.10479983232026828No Hit
CCTCTGAACTGTCCCCACCATTGCTGGGTTATGCAGTGTATTTTATATTG50.10479983232026828No Hit
GCATTCAAATGATCAAGGGGTTGGTATGGCTGCAGATAGGGATTCAACTC50.10479983232026828No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC50.10479983232026828No Hit
CGTCAGTATGTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATT50.10479983232026828No Hit
TCCATAATCCTGGATCAGTTCTGGACCCTCCCTTCTCTCCTCCAGGCCCA50.10479983232026828No Hit
ATCATGGCCTGATCGGGGTCCGGTCTGAGAAGGAAGGGAGGAGCCTGGGG50.10479983232026828No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA50.10479983232026828No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA50.10479983232026828No Hit
CCCTTAGGTGACTCTCCAATTGGCCATGTCTCCGATCTGTTTTCAAAGAA50.10479983232026828No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG50.10479983232026828No Hit
ACATAATGGATTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTT50.10479983232026828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTGC100.006871769145.000029
Produced by FastQC (version 0.11.5)